Blasticidin ant bl 1

Manufactured by InvivoGen

Sourced in United States

Blasticidin (ant-bl-1) is a lab equipment product. It is an antibiotic used for selection of transfected cells.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Puromycin ant pr 1, by InvivoGen (2 mentions) Zeocin ant zn 1, by InvivoGen (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions) Fluo 4 am, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Normocin ant nr 1, by InvivoGen (1 mentions) Taq dna ligase, by New England Biolabs (1 mentions) Phusion high fidelity dna polymerase, by New England Biolabs (1 mentions) T5 exonuclease, by New England Biolabs (1 mentions) Penicillin streptomycin, by Sartorius (1 mentions) Mc mir 2300, by Mirus Bio (1 mentions) T rextm 293 cells, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions) L glutamine, by Sartorius (1 mentions) Hygromycin, by Nacalai Tesque (1 mentions) Ryanodine, by Fujifilm (1 mentions) Poly methyl methacrylate pmma, by Merck Group (1 mentions) L glutamine, by Nacalai Tesque (1 mentions)

Spelling variants of this product

Blasticidin (577 citations) Ant-bl-1 (30 citations) Ant-bl (12 citations)

6 protocols using blasticidin ant bl 1

Comprehensive Chemical Reagents for Cell Studies

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The following chemicals and reagents were used in this study: torin1 (sc-396760, Santa Cruz, Dallas, TX), BafA1 (tlrl-baf1, InvivoGen, San Diego, CA), poly-l-lysine (P4707-50ML, MilliporeSigma), Lipofectamine 3000 (L3000008, Thermo Fisher Scientific), nucleofector kit T (VACA-1002, Lonza, Basel, Switzerland), polybrene (H9268-5G, MilliporeSigma), normocin (ant-nr-1, InvivoGen), puromycin (ant-pr-1, InvivoGen), blasticidin (ant-bl-1, InvivoGen), zeocin (ant-zn-1, InvivoGen, San Diego, CA), normal goat serum (NGS; ab7481, Abcam, Cambridge, United Kingdom), Phusion High-Fidelity DNA polymerase (M0530L, NEB, Ipswich, MA), T5 exonuclease (M0363S, NEB, Ipswich, MA), and Taq DNA ligase (M0208L, NEB, Ipswich, MA).

Shoemaker C.J., Huang T.Q., Weir N.R., Polyakov N.J., Schultz S.W, & Denic V. (2019). CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor. PLoS Biology, 17(4), e2007044.

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Tetracycline-Inducible T-REx-293 Cell Culture

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T-REx^TM-293 cells (Thermo-Scientific, Waltham, MA, USA) were grown at 37 °C, 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM, 01-055-1A, Biological Industries, Beit-Haemek, Israel) supplemented with 10% TET-System approved fetal bovine serum (04-005-1A, Biological Industries), 1% Penicillin-Streptomycin (03-031-1B, Biological Industries), 2 mM L-glutamine (03-020-1B, Biological Industries) and 5 mg/mL Blasticidin (ant-bl-1, InvivoGen, San Diego, CA, USA). Cells were not used above passage 30. Transfections were performed using TransIT-LT1 (MC-MIR-2300, Mirus Bio, Madison, WI, USA) transfection reagents, according to the manufacturer’s protocol. Tetracycline was added 18–24 h prior to experiments to achieve maximum saturating levels of plasmid expression.

Rhodes-Mordov E., Brandwine-Shemmer T., Zaguri R., Gutorov R., Peters M, & Minke B. (2023). Diacylglycerol Activates the Drosophila Light Sensitive Channel TRPL Expressed in HEK Cells. International Journal of Molecular Sciences, 24(7), 6289.

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Calcium Signaling Assay Protocol

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Dulbecco's Modified Eagle Medium (DMEM) (catalog no. 08488-55), l-glutamine (catalog no. 16948-04), and hygromycin (catalog no. 09287-84) were purchased from Nacalai Tesque Inc. (Kyoto, Japan). Fetal bovine serum (FBS; catalog no. 10437-028), penicillin and streptomycin (catalog no. 15140-122), Lipofectamine 2000 (catalog no. 11668), and fluo-4 AM (catalog no. F14217) were purchased from Thermo Fisher Scientific (Waltham, MA). Blasticidin (ant-bl-1) was purchased from InvivoGen (San Diego, CA). Collagen type 1 (catalog no. IFP9660) was purchased from the Research Institute for the Functional Peptides (Yamagata, Japan). Dox (catalog no. D9891), 2-APB (catalog no. D9754), and poly (methyl methacrylate) (PMMA; molecular weight [M_w] ∼15,000) (catalog no. 200336) were purchased from Sigma-Aldrich (St. Louis, MO). Thapsigargin (catalog no. 586005) was purchased from Merck (Darmstadt, Germany). Ryanodine (catalog no. 185-02821) was purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Europium (III) thenoyltrifluoroacetonate trihydrate (Eu-TTA) (catalog no. 21392-96-1) was purchased from Acros Organics (Pittsburgh, PA). The stocks of 1 mM fluo-4 AM in dimethyl sulfoxide (DMSO), 2 mM Thapsigargin in DMSO, 10 mM Ryanodine in distilled water, and 100 mM 2-APB in DMSO were stored at −20 °C until use.

Oyama K., Zeeb V., Yamazawa T., Kurebayashi N., Kobirumaki-Shimozawa F., Murayama T., Oyamada H., Noguchi S., Inoue T., Inoue Y.U., Nishino I., Harada Y., Fukuda N., Ishiwata S, & Suzuki M. (2022). Heat-hypersensitive mutants of ryanodine receptor type 1 revealed by microscopic heating. Proceedings of the National Academy of Sciences of the United States of America, 119(32), e2201286119.

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Generating p53 Knockdown Cells

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pRetroSuper-blasto-p53i plasmid, a kind gift from Professor Dagmar Kulms, Department of Dermatology, Technical University Dresden, was transfected to generate p53 knockdown cells. The transfected cells were selected using cell culture medium containing 5 μg/ml blasticidin (ant-bl-1) from InvivoGen. P53 expression of each clone was determined using a western blot. selected clones with the lowest p53 expression level were used in further experiments.

Nantajit D., Presta L., Sauter T, & Tavassoli M. (2022). EGFR-induced suppression of HPV E6/E7 is mediated by microRNA-9-5p silencing of BRD4 protein in HPV-positive head and neck squamous cell carcinoma. Cell Death & Disease, 13(11), 921.

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KRAS Mutant Flp-In T-REx 293 Cell Lines

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HEK293T and Flp-In T-REx 293 Cell Line (parental line; R78007; Thermo Fisher Scientific) were cultured in DMEM (high glucose, #12440-53; Gibco) supplemented with 10% FBS (#10500-64; Gibco), 2 mM L-glutamine (G7513; Sigma-Aldrich), 100 μg/ml Zeocin (ant-zn-1; InvivoGen), 15 μg/ml blasticidin (ant-bl-1; InvivoGen) at 37°C in a humidified 5% CO₂ atmosphere. The stable transfected Flp-In T-REx 293 cell lines (KRAS WT-APEX2, KRAS G12D-APEX2, KRAS G13D-APEX2, KRAS Q61H-APEX2, KRAS WT-GFP, KRAS G12D-GFP, KRAS G13D-GFP, KRAS Q61H-GFP, GFP only) were cultured in the same medium and selected by adding 100 μg/ml of hygromycin B (ant-hg-1; InvivoGen) to the media. For the induction of the stable transfected Flp-In T-REx 293 cell lines, 1 μg/ml tetracycline was added to the media.

Damianou A., Liang Z., Lassen F., Vendrell I., Vere G., Hester S., Charles P.D., Pinto-Fernandez A., Santos A., Fischer R, & Kessler B.M. (2024). Oncogenic mutations of KRAS modulate its turnover by the CUL3/LZTR1 E3 ligase complex. Life Science Alliance, 7(5), e202302245.

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Antibody-based detection of HIV proteins

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The following antibodies have been used in the study: MATR3 (A300-590A, Bethyl Laboratories, 1:2,000 dilution for IB), HIV-1 p55 and p24 (HIV-1 p24 [1941], sc-65462, Santa Cruz, or anti-HIV-1 p55⁺ p24⁺ p17 antibody ab63917, Abcam, 1:200). Hsp90 (catalogue number ALX-804-808, Enzo Life Sciences), PARP (catalogue number ALX-210-302, Enzo Life Sciences), β-actin HRP (Sigma, AC-15, 1:10,000), PSF (B92 Sigma P2860, 1:1,000), cyclin T1 (C-20-SC-8128, 1:200), polypyrimidine tract binding protein PTB (rabbit polyclonal produced in-house, 1:1,000), flag tag (Sigma, 1:5,000), and vimentin (Cell Signaling, 1:1,000).
Other reagents included Histopaque-1077 (10771, Sigma), Polybrene (Sigma-H9268), phytohemagglutinin (PHA L1668-5MG, Sigma), interleukin-2 (H7041, Sigma), TNF-α (T0157, Sigma), puromycin (ant-pr-1, InvivoGen), blasticidin (ant-bl-1, InvivoGen), SAHA (SML0061, Sigma), disulfiram (D2950000, Sigma), romidepsin (S3020, Selleckchem), and JQ1 (2091-1, BioVision). IngenolB was kindly donated by Luiz F. Pianowski, Kyolab/Amazônia Fitomedica-mentos, Valinhos, Sao Paulo, Brazil. Human CD3 (IMI1304) and CD28 (IMI1376) antibodies were obtained from Analis (Belgium).

Sarracino A., Gharu L., Kula A., Pasternak A.O., Avettand-Fenoel V., Rouzioux C., Bardina M., De Wit S., Benkirane M., Berkhout B., Van Lint C, & Marcello A. (2018). Posttranscriptional Regulation of HIV-1 Gene Expression during Replication and Reactivation from Latency by Nuclear Matrix Protein MATR3. mBio, 9(6), e02158-18.

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