Ds qi1nc

The preparations were examined using a Nikon Eclipse Ni microscope equipped with the appropriate filter cubes to differentiate the fluorochromes employed. The images were recorded using a Nikon DS-Qi1Nc digital camera and NIS Elements software BR 4.20.01 (Nikon Instruments Europe BV, Amsterdam, Netherlands). Slight adjustments to contrast and brightness were made using Corel Photo Paint, whereas the figure panels were prepared using Corel Draw (Corel Photo Paint and Corel Draw, Ottawa, ON, Canada).

Preparations were examined on a Nikon Eclipse Ni microscope equipped with the appropriate filter cubes to distinguish the fluorochromes employed. The images were recorded with a Nikon DS-Qi1Nc digital camera and NIS Elements software BR 4.20.01 (Nikon Instruments Europe BV, Amsterdam, Netherlands). Enteric neuron counts were performed at ×40 magnification. Slight adjustments to contrast and brightness were made using Corel Photo Paint, whereas the figure panels were prepared using Corel Draw (Corel Photo Paint and Corel Draw, Ottawa, ON, Canada).

The slides were observed using a Nikon Eclipse Ni microscope equipped with the appropriate filter cubes to differentiate the fluorochrome employed. Images were recorded using a Nikon DS-Qi1Nc digital camera and NIS elements software BR 4.20.01 (Nikon Instruments Europe BV, Amsterdam, Netherlands). The figure panels were prepared using Corel Draw (Corel Photo Paint and Corel Draw, Ottawa, ON, Canada).
The immunoreactivity of the antibodies was evaluated by an expert pathologist and its distribution in the epidermis and the cellular localization (nuclear, membranous, cytoplasmic) were reported. The intensity of the expression was evalutated semiquantitatively in pictures acquired using the same exposure times, as faint, moderate and bright.

The specimens were examined using a Nikon Eclipse Ni microscope and images were taken using a Nikon DS-Qi1Nc digital camera and NIS Elements software BR 4.20.01 (Nikon Instruments Europe BV, Amsterdam, The Netherlands). Minor adjustments to contrast and brightness were made using Corel Photo Paint, while figure panels were prepared using Corel Draw (Corel Photo Paint and Corel Draw, Ottawa, ON, Canada). The 20× objective was used for morphometric evaluation. In the gastric mucosa, the area occupied by OPs-IR in 4.1 mm² (0.410 × 10 fields) was measured by binarization (described above). In addition, the number of GHR, NPY and SOM IRs in 4.1 mm² were counted in the gastric mucosa.
For each experimental group (CTR, BP 5% and BP 10%), the values obtained for OPs-IR area and the number of EECs were corporate and the means were calculated. The results were expressed as mean ± standard deviation (SD). The data were analyzed by one-way ANOVA (Graph Prism 4, GraphPad Software version 4.01, Inc., La Jolla, CA, USA). The experimental group was considered as the main effect. In addition, the means were then separated using the Tukey-HSD test. A p ≤ 0.05 was considered statistically significant.

The following method was used to characterize the area occupied by the OPs immunoreactive (-IR) cells in the gastric mucosa, which was previously described by Mazzoni et al. [50 ]. Briefly, after scanning the preparations with a digital camera (Nikon DS-Qi1Nc, Nikon Instruments Europe BV, Amsterdam, The Netherlands ) using the 20× objective and the NIS Elements BR 4.20.01 software (Nikon Instruments Europe BV, Amsterdam, The Netherlands), binarization was performed in a selected region of the gastric mucosa. This method allows the evaluation of the area occupied by the OPs-IR cells within the considered area. Gastric morphometric evaluations were performed by two investigators in a blinded fashion.

The cellular uptake of NLC dispersions was determined after seeding 20 × 10³ cells/well on 24-well plates. The CRN-NLC samples were incubated with LNCaP cell for 24 h at different concentrations. After 24 h, the cell medium was removed and rinsed with a phosphate buffer solution (3 times). The cells were left in the dark for 15 min after the addition of 1 ml paraformaldehyde (4%) to fix them on the cover slips and subsequently mounted on glass slides with a vectashield medium enclosing DAPI. The uptake of CRC-NLCs was determined using the CRC fluorescence intensity which excites at 425 nm and emits at 530 nm [17 (link)]. Fluorescent images were collected with a Nikon ECLIPSE 90i microscope coupled to a Nikon (DS-Qi1Nc) digital camera using the NIS-Elements Advanced Research software. For the imaging studies, an oil immersion CFI Plan Apochromat VC 60 × N2 was used.