Mouse igg2a isotype control c1

TIL2 cells were cultured tumor infiltrating lymphocytes established from the same patient from which Mel2 cells were established, as described previously.^{13 (link)} Briefly, melanoma tumor digests were cultured in a 24-well plate containing 2 ml of RPMI1640 supplemented with 10% human serum, antibiotics and recombinant human IL-2 (6000 IU/ml, Novartis). Five days after initiation, half of the medium was replaced with fresh complete medium containing IL-2; this was repeated every 2 to 3 days thereafter as needed. The recognition of Mel2 cells by TIL2 cells was confirmed by examining IFN-γ production from TIL2 cells (2 × 10⁵) cocultured with Mel2 cells (1 × 10⁵) with 10 μg/ml of anti-HLA-ABC Ab (W6/32, BioXcell, West Lebanon, NH, USA) or mouse IgG2a isotype control (C1.18.4, BioXcell).