Anti mouse ecadherin

The antibodies used were as follows:
Anti-mouse Etv2 (Abcam, EPR5229(2); western blotting 1:100)
Anti-mouse Erg (Abcam, 9FY; western blotting 1:100)
Anti-mouse Fli1 (Abcam, ab15289; western blotting 1:100)
Anti-mouse CD31 (Biolegend clone 390; flow cytometry 1:2,000)
Anti-mouse VE-Cadherin (Biolegend clone BV13; flow cytometry 1:500)
Anti-mouse Vegfr2 (DC101; flow cytometry 1:500)
Anti-mouse CD62e (BD Biosceinces, clone 10E9.6; flow cytometry 1:500)
Anti-mouse Itgb1 (BD Biosciences, clone 18/CD29; flow cytometry 1:500)
Anti-mouse Meca32 (BD Biosciences, clone MECA-32; flow cytometry 1:500)
Anti-mouse Tie2 (Biolegend, clone Tek4; flow cytometry 1:500)
Anti-mouse ECadherin (BD Biosciences, Clone 36/E-Cadherin; flow cytometry 1:500)
Anti-mouse CD34 (BD Biosciences, Clone RAM34; flow cytometry 1:500)
Anti-mouse Vcam (BD Biosciences, Clone 429 MVCAM.A; flow cytometry 1:500)
Anti-mouse Sca1 (Biolegend, clone D7; flow cytometry 1:500)
Anti-mouse CD41 (Biolegend, clone MWReg30; flow cytometry 1:500)
Anti-mouse CD24 (BD Biosciences clone M1/69; flow cytometry 1:500)
Anti-mouse VE-Cadherin (R+D AF1002; IF 1:100)
Anti-mouse CD31 (Biocare, Clone Mec13.3; IF 1:100)
Uncropped western blotting images are shown in Supplementary Fig. 5.

Freshly cut FFPE sections (5 μm) were made of the indicated anatomical sections and stored with desiccants at 4°C. Subsequently, sections were processed and analyzed using the RNAscope Multiplex Fluorescent v2 Assay (Advanced Cell Diagnostics USA-ACDbio) combined with immunofluorescence. Briefly, sections were processed following ACDbio recommendations for FFPE sample preparation and pretreatment using 15-min target retrieval and 25-min Protease Plus digestion using the RNAscope HybEZ oven for all incubations. An RNAscope C1 probe (OcIL8) to rabbit chemokine (C-X-C motif) ligand 8 (CXCL8) was developed and used to stain intestinal sections for CXCL8 mRNA expression. The C1 probe was detected with Opal 570 dye (Akoya Biosciences) diluted 1:1,000 in Multiplex TSA buffer (ACDbio). Sections were also stained with DAPI (2 μg/ml), anti-Shigella labeled with FITC (1/1,000; Virostat), and anti-mouse E-cadherin (1/100) (catalog no. 610181, BD Biosciences). Slides were imaged using a Nikon Ti Eclipse equipped with a spinning disk confocal scanner unit (Yokogawa CSU-Xu1) and EMCCD (Andor iXon3) camera for high-magnification images. Slides were imaged using a wide-field Zeiss Axioplan 2 microscope through the MetaMorph imaging system for RNAscope signal quantification.