Cells were fixed in situ with 2.5% glutaraldehyde in 0.1 M phosphate buffer at room temperature (RT) for 20 min. Samples were rinsed and then post-fixed with 1% osmium tetroxide and 1% potassium ferrocyanide in 0.1 M cacodylate buffer for 1 h at RT to enhance the staining of cytoplasmic membranes. Cells were rinsed with distilled water, embedded in epoxy resin, sectioned, and examined with a Philips CM12 transmission electron microscope equipped with an Olympus SIS CCD camera. Images were acquired randomly by a blinded investigator. We counted the total number of mitochondria (n > 150) in contact or not with ER in more than 10 different micrographs obtained from two different experiments. Data are presented as a percentage of mitochondria in contact with ER.
Sis ccd camera
Manufactured by Olympus
The SIS CCD camera is a high-performance charge-coupled device (CCD) camera designed for scientific and industrial applications. It features a large, high-resolution CCD sensor that captures detailed, low-noise images. The camera is capable of capturing images with high sensitivity and dynamic range, making it suitable for a variety of imaging tasks.
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Lab products found in correlation
2 protocols using sis ccd camera
1
Mitochondria-ER Contact Quantification
2
Transmission Electron Microscopy Protocol for Mitochondrial Morphometrics
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Cells were fixed in situ with 1.6 % glutaraldehyde in 0.1 M phosphate buffer at room temperature (RT) and stored overnight at 4 °C. Samples were rinsed in the same buffer and then postfixed with 1 % osmium tetroxide and 1 % potassium ferrocyanide in 0.1 M cacodylate buffer for 1 h at RT to enhance the staining of cytoplasmic membranes. Cells were rinsed with distilled water, embedded in epoxy resin, sectioned, and examined with a Philips CM12 transmission electron microscope equipped with an Olympus SIS CCD camera. The area of the mitochondria of cells was calculated as an ellipse (0.785ab; n.2, 50 mitochondria per experiment; mean ± SD).
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