Penicillin g

Manufactured by Atlanta Biologicals

Sourced in United States

Penicillin G is a laboratory product used as a broad-spectrum antibiotic. It is a naturally occurring substance produced by the Penicillium fungi. Penicillin G is commonly used in research and scientific applications to inhibit the growth of various gram-positive and gram-negative bacteria.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (3 mentions) Streptomycin, by Atlanta Biologicals (2 mentions) Dulbecco modified eagle medium (dmem), by Atlanta Biologicals (2 mentions) Streptomycin sulfate, by Thermo Fisher Scientific (1 mentions) Tc 100 medium, by Thermo Fisher Scientific (1 mentions) Dh10b escherichia coli cells, by Thermo Fisher Scientific (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Calu 3 cells, by American Type Culture Collection (1 mentions) Bhk 21 cells, by American Type Culture Collection (1 mentions) Penicillin g, by American Type Culture Collection (1 mentions) Let 1 cells, by BEI Resources (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Penicillin g, by Corning (1 mentions) Galaxy 48r co2 incubator, by Eppendorf (1 mentions)

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Penicillin (39 citations)

4 protocols using penicillin g

Sf9 Cell Culture and Baculovirus Production

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The Spodoptera frugiperda-derived insect cell line Sf9 (clonal isolate 9 from cell line IPLB-SF21-AE) was purchased from ATCC and cultured at 27°C in TC-100 medium (Invitrogen) supplemented with 10% fetal bovine serum (Atlanta Biologicals), penicillin G (60 μg/ml), streptomycin sulfate (200 μg/ml), and amphotericin B (0.5 μg/ml).
DH10B Escherichia coli cells with helper plasmid pMON7124, encoding a transposase, were purchased from Invitrogen. Recombinant bacmids were maintained in DH10B cells (Invitrogen).
Third instar Trichoplusia ni larvae were purchased from Benzon Research (Carlisle, PA). Larvae were reared with artificial diet (Southland Products, Inc.) in a 27°C chamber with a 12 h/12 h light/dark cycle and infected shortly after they reached 4^th instar.

Ishimwe E., Hodgson J.J, & Passarelli A.L. (2015). Expression of the Cydia pomonella granulovirus matrix metalloprotease enhances Autographa californica multiple nucleopolyhedrovirus virulence and can partially substitute for viral cathepsin. Virology, 481, 166-178.

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Cell Line Maintenance Protocols

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HEK293T, HeLa, and HeLa-hACE2 (obtained from Ed Campbell, Loyola University Chicago) cells were maintained in Dulbecco’s modified Eagle medium (DMEM)-10% fetal bovine serum (FBS) (containing 10 mM HEPES, 100 nM sodium pyruvate, 0.1 mM nonessential amino acids, 100 U/ml penicillin G, and 100 μg/ml streptomycin and supplemented with 10% FBS; Atlanta Biologicals). Calu-3 cells (obtained from Paul McCray, University of Iowa) were maintained in MEM-20% FBS (MEM supplemented with 20% FBS, 100 U/ml penicillin G, and 100 μg/ml streptomycin). All cell lines were cultured in a 5% CO₂ incubator at 37°C.

Qing E., Kicmal T., Kumar B., Hawkins G.M., Timm E., Perlman S, & Gallagher T. (2021). Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains. mBio, 12(4), e01590-21.

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Cell Culture Maintenance Protocols

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HEK293T (ATCC), HeLa (ATCC), and HeLa-mCEACAM (77 (link),
78 (link)) cells were maintained in DMEM−10% FBS
medium (Dulbecco’s modified Eagle medium [DMEM] containing 10 mM HEPES,
100 nM sodium pyruvate, 0.1 mM nonessential amino acids, 100 U/ml penicillin
G, and 100 μg/ml streptomycin, and supplemented with 10% fetal bovine serum
[FBS] [Atlanta Biologicals]). BHK-21 cells (ATCC) were maintained in DMEM−5% FBS
medium. LET-1 cells (BEI Resources) (79 (link)) were
maintained in DMEM−10% FBS medium lacking HEPES, sodium pyruvate and nonessential
amino acids. Calu3 cells (ATCC) were maintained in MEM−20% FBS medium (minimum
essential medium [MEM] supplemented with 20% FBS, 100 U/ml penicillin G, and
100 μg/ml streptomycin). DBT cells (80 (link), 81 (link)) were maintained in MEM−5%
FBS medium (MEM supplemented with 5% FBS, 10% tryptose-phosphate broth, 26.8 mM
sodium bicarbonate, 2 mM l-glutamine, 100 U/ml penicillin G, and
100 μg/ml streptomycin). All cell lines were cultured in a 5% CO₂incubator at 37°C.

Qing E., Hantak M., Perlman S, & Gallagher T. (2020). Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection. mBio, 11(1), e02764-19.

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Culturing Retinal Cell Types in Hypoxia

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Retinal pigmented epithelial (RPE) cells from human were generously provided by Dr. Nader Rahimi and were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Corning, Tewkesbury, MA) containing 10% calf serum (Hyclone, Logan UT, USA), 2 mM L-glutamine, and 100 units/mL penicillin G and 100 µg/mL streptomycin (Corning, Tewkesbury, MA). Rat Retinal Endothelial cells (REC), generously provided by Dr. Sayon Roy, and vascular smooth muscle cells, isolated from neonatal rat aortas as described [60 (link)], were maintained in DMEM containing 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA, USA), 2 mM L-glutamine, and 100 units/mL penicillin G and 100 µg/mL streptomycin. Hypoxia conditions were induced by maintaining the cells in an environment composed of 1% pO₂ in a Galaxy 48R CO₂ Incubator (Eppendorf North America, Hauppague, NY, USA) for the indicated treatment times while normoxic cells were maintained in a humidified cell culture incubator at 20% pO₂.

Buczek-Thomas J.A., Rich C.B, & Nugent M.A. (2019). Hypoxia Induced Heparan Sulfate Primes the Extracellular Matrix for Endothelial Cell Recruitment by Facilitating VEGF-Fibronectin Interactions. International Journal of Molecular Sciences, 20(20), 5065.

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