Deltavision microscopy imaging system

Manufactured by GE Healthcare

The DeltaVision microscopy imaging system is a high-performance microscope designed for advanced fluorescence imaging. It is capable of capturing high-resolution images of biological samples, providing researchers with a powerful tool for visualizing cellular structures and dynamics.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Szx10 fluorescent stereo microscope, by Olympus (1 mentions) Ix83 inverted fluorescence microscope, by Olympus (1 mentions) Ix71 inverted fluorescence microscope, by Olympus (1 mentions)

Close spelling variants of this product

Deltavision Elite Microscopy Imaging Systems DeltaVision microscopy imaging system v. 3.70 DeltaVision microscopy system DeltaVision imaging system DeltaVision system

2 protocols using deltavision microscopy imaging system

Immunostaining of Embryonic Stem Cells

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EpiScs and ESCs were fixed with 4% paraformaldehyde in PBS. After permeabilization in 1% Triton X-100/PBS for 30 min, immunostaining was performed using the following primary antibodies: Oct4 (Santa Cruz Biotechnology, Cat# sc-8628, RRID: AB_653551), alpha-Tubulin (Abcam, Cat# ab15246, RRID: AB_301787), and GFP (MPI-CBG, protein facility). Secondary antibodies used were Alexa Fluor 488/594 anti-mouse IgG, anti-rabbit, or anti-goat IgG (Invitrogen). DAPI (Invitrogen) was used for staining the nuclei. Images were taken using a DeltaVision microscopy imaging system (GE Life Sciences).

Ding L., Paszkowski-Rogacz M., Winzi M., Chakraborty D., Theis M., Singh S., Ciotta G., Poser I., Roguev A., Chu W.K., Choudhary C., Mann M., Stewart A.F., Krogan N, & Buchholz F. (2015). Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells. Cell systems, 1(2), 141-151.

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Microscopy-based Biofilm and Single Cell Analysis

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Movies and snapshots were acquired with an IX71 inverted fluorescence microscope (Olympus), IX83 inverted fluorescence microscope (Olympus) or DeltaVision microscopy imaging system (GE Healthcare). Biofilm movies were taken from the bottom with a 2.5x objective lens (MPLFLN 2.5x/0.08, Olympus) to film the whole biofilm or a 10x objective lens (UPLFLN 10x/0.3, Olympus) to zoom in on certain regions. Images were taken every 40 min. Single cell movies were taken with a 100x objective lens (UPLFLN 100x/1.3, Olympus) while growing 10-fold diluted cell cultures on an MSgg pad at 30°C. Top-view of biofilms was acquired by a Retiga 2000R digital camera (QImaging) via an SZX10 fluorescent stereomicroscope (Olympus).

Zhang F., Kwan A., Xu A, & Süel G.M. (2015). A Synthetic Quorum Sensing System Reveals a Potential Private Benefit for Public Good Production in a Biofilm. PLoS ONE, 10(7), e0132948.

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