Anti rpa70

Cells were fixed 24 hours after transfection. Fixed cells were processed for immunofluorescence. The following antibodies were used: anti-FLAG (Sigma, F7425 or F1804), anti-RPA70 (Cell Signaling, 2267), anti-RPA32 pS33 (Abcam, ab87278), anti-BLM (Santa Cruz, SC-7790), anti-Rad51 (Santa Cruz SC-8349), anti-RecQL4 (Abcam, ab34800), anti-gamma H2AX (Cell Signaling, 2577), and anti-TRF1 (Santa Cruz, SC-6165). Primary antibodies were diluted 1:1000, except the in-house ATRIP antibody, which was diluted 1:20. Secondary antibodies (Alexa Fluor, Invitrogen) were diluted 1:2000.

Primary antibodies were purchased as follows: anti-phospho-(Ser296)-CHK1 (rabbit polyclonal, #2349S), anti-phospho-(Ser317)-CHK1 (rabbit polyclonal, #2344S) and anti-RPA70 (rabbit polyclonal, #2267) were from Cell Signaling (Cell Signaling, Danvers, MA, USA); anti-NEK9 (mouse monoclonal, #sc-100401), anti-CHK1 (mouse, monoclonal, #sc-8408) and anti-OctA (FLAG) (mouse, monoclonal, #sc-51590) were from Santa Cruz Biotechnology (Dallas, TX, USA); anti-γ-H2AX (mouse monoclonal, #05–636) and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (mouse monoclonal, #MAB374) were from Millipore (Temecula, CA, USA). Mouse and rabbit horseradish peroxidase-conjugated secondary antibodies (anti-mouse, #7076S; anti-rabbit, #7074S) were from Cell Signaling. Normal mouse immunoglobulin G (IgG) (N103) was from Calbiochem, Millipore. Anti-mouse IgG-Alexa Fluor 488 was from Invitrogen. Anti-rabbit IR dye (800) (#926–32213) was from LI-COR Biosciences.