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Vinculin v284

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Vinculin (V284) is a laboratory reagent used in cell biology research. It functions as a cytoskeletal protein involved in cell-cell and cell-matrix junctions.

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Lab products found in correlation

Bromodeoxyuridine (brdu), by Roche (2 mentions) P53 cm5, by Leica (2 mentions) Gfp jl 8, by Takara Bio (2 mentions) Lamin b1 12586, by Cell Signaling Technology (2 mentions) α tubulin b 5 1 2, by Merck Group (2 mentions) Ha 3f10, by Roche (2 mentions) C myc 9e10 and c33, by Santa Cruz Biotechnology (2 mentions) Ki 67 sp6, by Lab Vision (2 mentions) β actin ac 15, by Lab Vision (2 mentions) N myc 9405, by Takara Bio (2 mentions) Parp1 9532, by Cell Signaling Technology (2 mentions) P53 do 1, by Santa Cruz Biotechnology (2 mentions) Cd8 53 6.7, by BD (1 mentions)

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Vinculin (439 citations)

3 protocols using vinculin v284

1

Antibody Information for Western Blot

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Antibodies used in this study include N-Myc (OP-13; 1:1000) from EMD Biosciences; p53 (DO-1; 1:1000), and c-Myc (9E10 and C33; 1:500) from Santa Cruz Biotechnology; Ki-67 (SP6; 1:500) from Lab Vision; β-actin (AC-15; 1:10,000), Vinculin (V284; 1:1000), α-Tubulin (B-5-1-2; 1:4000) and Flag (M2; 1:1000) from Sigma; p53 (CM5; 1:1000) from Leica Microsystems; HA (3F10; 1:1000) and BrdU (1:500) from Roche Applied Science; GFP (JL-8; 1:1000) from Clontech; N-Myc (9405; 1:500), Lamin B1 (12586; 1:1000), and Parp1 (9532; 1:1000) from Cell Signaling; For IHC, HAUSP (1:500) Bethyl; For westerns, HAUSP antibody as described before5 (link) (1:1000). All primary antibodies were diluted in 5% (w/v) nonfat dry milk in Tris-buffered saline with 0.1% Tween-20, except N-Myc (9405) which was diluted in 5% (w/v) BSA in Tris-buffered saline with 0.1% Tween-20.
Tavana O., Li D., Dai C., Lopez G., Banerjee D., Kon N., Chen C., Califano A., Yamashiro D.J., Sun H, & Gu W. (2016). HAUSP deubiquitinated and stabilizes N-Myc in neuroblastoma. Nature medicine, 22(10), 1180-1186.
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2

Multicolor Flow Cytometry Antibodies

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Purified antibodies against arginase I (clone19), iNOS (54/iNOS), gp91phox (53/gp91), and fluorochrome-conjugated antibodies against CD8 (53-6.7), CD11b (M1/70), CD44 (IM7), CD45.1 (A20), CD45.2 (104), CD49f (GoH3), CD69 (H1.2F3), Gr-1 (RB6-8C5), XCR1 (ZET), CD103 (2E7), IFNγ (XMG1.2) and Ki-67 (16A8) were obtained from Becton Dickinson (San Jose, CA) or Biolegend (San Diego, CA). Antibodies against β-actin (AC-74) and vinculin (V284) were from Sigma-Aldrich (St. Louis, MO) and anti-p84 (5E10) from Abcam (Cambridge, MA). Polyclonal antibodies against NFκB-p65 (D14E1) and Jagged1 (28H8) were obtained from Cell Signaling Technologies (Beverly, MA), while anti-Jagged2 (H-143) was from Santa Cruz Biotechnologies.
Sierra R.A., Trillo-Tinoco J., Mohamed E., Yu L., Achyut B.R., Arbab A., Bradford J.W., Osborne B.A., Miele L, & Rodriguez P.C. (2017). Anti-Jagged immunotherapy inhibits MDSCs and overcomes tumor-induced tolerance. Cancer research, 77(20), 5628-5638.
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3

Antibody Information for Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study include N-Myc (OP-13; 1:1000) from EMD Biosciences; p53 (DO-1; 1:1000), and c-Myc (9E10 and C33; 1:500) from Santa Cruz Biotechnology; Ki-67 (SP6; 1:500) from Lab Vision; β-actin (AC-15; 1:10,000), Vinculin (V284; 1:1000), α-Tubulin (B-5-1-2; 1:4000) and Flag (M2; 1:1000) from Sigma; p53 (CM5; 1:1000) from Leica Microsystems; HA (3F10; 1:1000) and BrdU (1:500) from Roche Applied Science; GFP (JL-8; 1:1000) from Clontech; N-Myc (9405; 1:500), Lamin B1 (12586; 1:1000), and Parp1 (9532; 1:1000) from Cell Signaling; For IHC, HAUSP (1:500) Bethyl; For westerns, HAUSP antibody as described before5 (link) (1:1000). All primary antibodies were diluted in 5% (w/v) nonfat dry milk in Tris-buffered saline with 0.1% Tween-20, except N-Myc (9405) which was diluted in 5% (w/v) BSA in Tris-buffered saline with 0.1% Tween-20.
Tavana O., Li D., Dai C., Lopez G., Banerjee D., Kon N., Chen C., Califano A., Yamashiro D.J., Sun H, & Gu W. (2016). HAUSP deubiquitinated and stabilizes N-Myc in neuroblastoma. Nature medicine, 22(10), 1180-1186.
+ Open protocol
+ Expand

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