Anti interferon ifn γ fitc

Manufactured by BD

Anti-interferon (IFN)-γ-FITC is a fluorescently-labeled antibody that binds to and detects the presence of interferon-gamma, a key cytokine involved in immune response. This product is designed for use in flow cytometry, immunohistochemistry, and other laboratory applications where the identification and quantification of interferon-gamma is required.

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2 protocols using anti interferon ifn γ fitc

Multiparameter Flow Cytometry Analysis

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The following anti-human monoclonal antibodies were used for surface phenotype and intracellular cytokine staining: anti-CD4-fluorescein isothiocyanate (FITC); anti-CD4-phycoerythrin (PE); anti-CD161-PE; anti-CCR6-PE; anti-CD45RO-PE; anti-CD147-peridinin chlorophylla protein cyanine 5.5 dies (Percp–cy5.5); anti-interferon (IFN)-γ-FITC (all from BD Biosciences); and anti-IL-17A-allophycocyanin (APC; eBiosciences). Anti-mouse monoclonal antibodies included: anti-CD4-Percp; anti-IL-17A-APC; and anti-IFN-γ-FITC (all from BD Biosciences). Appropriately conjugated IgG antibodies were used as isotype controls. Cells were acquired on a FACSCalibur flow cytometer (BD Biosciences) and analyzed using Cell Quest software (BD Bioscience) and FlowJo 7.6.1 software (Tree Star).

Miao J., Zhang K., Zheng Z., Zhang R., Lv M., Guo N., Xu Y., Han Q., Chen Z, & Zhu P. (2020). CD147 Expressed on Memory CD4+ T Cells Limits Th17 Responses in Patients With Rheumatoid Arthritis. Frontiers in Immunology, 11, 545980.

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Gemcitabine-Modulated NK Cell Activation

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HPC-NK cells were stimulated for 4 h with K562 or SKOV-3 cells that were 48 h pre-treated with/without 8.8 nM gemcitabine at an effector-to-target (E:T) ratio of 1.5:1 in the presence of CD107a-PE-Cy7 (Biolegend, 328618), and brefeldin A (added after 1 h, BD Biosciences, 555029). Optionally, anti-DNAX Accessory Molecule-1 (DNAM-1) (mouse IgG1, Biolegend, 338302), and/or anti-NKG2D (mouse IgG1, Biolegend, 320813) blocking antibodies or isotype control (mouse IgG1, BioXcell, BE0083) were added at 10 μg/ml. After 4 h, surface staining was performed using CD56-BV510 (Biolegend, 318340), and intracellular staining using anti-interferon (IFN)γ-FITC (BD Biosciences, 554700) and anti-perforin-PE (Biolegend, 308106) using a fixation/permeabilization kit (eBioscience, 00–5521-00) and permeabilization buffer (eBioscience, 00–8333) following manufacturer’s instructions. Dead cells were excluded using fixable viability dye eFluor780 (eBioscience, 65–0865-14) staining. Unstimulated cells were used for gating on CD107a and IFNγ positive cells.

Van der Meer J.M., de Jonge P.K., van der Waart A.B., Geerlings A.C., Moonen J.P., Brummelman J., de Klein J., Vermeulen M.C., Maas R.J., Schaap N.P., Hoogstad-van Evert J.S., Ottevanger P.B., Jansen J.H., Hobo W, & Dolstra H. (2021). CD34+ progenitor-derived NK cell and gemcitabine combination therapy increases killing of ovarian cancer cells in NOD/SCID/IL2Rgnull mice. Oncoimmunology, 10(1), 1981049.

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