The reverse-phase HPTLC analysis was performed using the CAMAG HPTLC instrument (CAMAG, Muttenz, Switzerland). The estimation of caffeine was performed on 10 × 20 cm glass plates precoated with reverse-phase silica gel 60 F254S plates (E-Merck, Darmstadt, Germany). The samples to the reverse-phase TLC plates were spotted as the 6 mm bands utilizing a CAMAG Automatic Sampler 4 (ATS4) applicator (CAMAG, Geneva, Switzerland). The sample applicator was fitted with CAMAG Microliter Syringe (Hamilton, Bonaduz, Switzerland). The application rate for caffeine estimation was constant at 150 nL s−1. The TLC plates were developed in an Automatic Developing Chamber 2 (ADC 2) (CAMAG, Muttenz, Switzerland) to a distance of 80 mm using EtOH-water (55:45, v v−1) greener mobile phase. The development chamber was saturated with vapors of EtOH-water (55:45, v v−1) for 30 min at 22 °C. Caffeine was detected using a wavelength of 275 nm at a scanning rate of 20 mm s−1 and slit size set to 4 × 0.45 mm.
60 f254s plates
Manufactured by Merck Group
Sourced in Germany
The 60 F254S plates are thin-layer chromatography (TLC) plates manufactured by Merck Group. These plates are coated with a fluorescent indicator silica gel, which allows for the visualization of separated compounds under ultraviolet (UV) light. The plates are designed for analytical and preparative TLC applications.
Automatically generated - may contain errors
2 protocols using 60 f254s plates
1
Reverse-Phase HPTLC Quantification of Caffeine
2
Simultaneous RP-HPTLC-Densitometry Analysis of 6-SHO and 6-GIN
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Simultaneous RP-HPTLC-densitometry analysis of 6-SHO and 6-GIN was carried out using the following instrumentation and analytical conditions:
HPTLC apparatus: CAMAG TLC system (CAMAG, Muttenz, Switzerland)
Software: WinCAT’s (version 1.4.3.6336, CAMAG, Muttenz, Switzerland)
Syringe for sample application: CAMAG microliter Syringe (Hamilton, Bonaduz, Switzerland)
TLC plate: 10 × 20 cm glass backed plates pre-coated with RP silica gel 60 F254S plates (E-Merck, Darmstadt, Germany)
Sample applicator: CAMAG Linomat-V (CAMAG, Muttenz, Switzerland)
Gas for sample application: nitrogen
Development chamber: CAMAG automatic developing chamber 2 (ADC2) (CAMAG, Muttenz, Switzerland)
TLC scanner: CAMAG TLC scanner-III (CAMAG, Muttenz, Switzerland)
Stationary phase: 10 × 20 cm glass backed plates pre-coated with RP silica gel 60 F254S plates (E-Merck, Darmstadt, Germany)
Mobile phase for 6-SHO and 6-GIN: ethanol:water (6.5:3.5 v/v)
Saturation time: 30 min at 22 °C
Development distance on plate: 80 mm
Development mode: linear ascending mode
Sample application rate: 150 nL/s
Densitometry of scanning mode: absorbance/reflectance.
Scanning wavelength for 6-SHO and 6-GIN: 200 nm
HPTLC apparatus: CAMAG TLC system (CAMAG, Muttenz, Switzerland)
Software: WinCAT’s (version 1.4.3.6336, CAMAG, Muttenz, Switzerland)
Syringe for sample application: CAMAG microliter Syringe (Hamilton, Bonaduz, Switzerland)
TLC plate: 10 × 20 cm glass backed plates pre-coated with RP silica gel 60 F254S plates (E-Merck, Darmstadt, Germany)
Sample applicator: CAMAG Linomat-V (CAMAG, Muttenz, Switzerland)
Gas for sample application: nitrogen
Development chamber: CAMAG automatic developing chamber 2 (ADC2) (CAMAG, Muttenz, Switzerland)
TLC scanner: CAMAG TLC scanner-III (CAMAG, Muttenz, Switzerland)
Stationary phase: 10 × 20 cm glass backed plates pre-coated with RP silica gel 60 F254S plates (E-Merck, Darmstadt, Germany)
Mobile phase for 6-SHO and 6-GIN: ethanol:water (6.5:3.5 v/v)
Saturation time: 30 min at 22 °C
Development distance on plate: 80 mm
Development mode: linear ascending mode
Sample application rate: 150 nL/s
Densitometry of scanning mode: absorbance/reflectance.
Scanning wavelength for 6-SHO and 6-GIN: 200 nm
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