The 3,4′,5-trihydroxy-trans-stilbene (resveratrol; purity ≥ 99%), L-glutamic acid, Dulbecco’s modified Eagle’s medium (DMEM), and fetal bovine serum (FBS) were purchased form Sigma-Aldrich (St. Louis, MO, USA). The 3-(4,5-dimetylthiazol-2-yl)- 2,5-diphenyltetra-zoliumbromide (MTT) was purchased from Bio Basic (Markham, ON, Canada). The 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) was obtained from Molecular Probes (Eugene, OR, USA). The Trizol reagent was purchased from Invitrogen (Carlsbad, CA, USA). The penicillin–streptomycin solution was purchased from Gibco (Waltham, MA, USA). The annexin V-FITC apoptosis detection kit was purchased from BioLegend (San Diego, CA, USA). The RT Pre-Mix and qPCR Master Mix solutions were obtained from Bioneer (Daejeon, South Korea). The chromatin immunoprecipitation (ChIP) assay kit was purchased from Merck (Kenilworth, NJ, USA).
Resveratrol 3 4 5 trihydroxy trans stilbene
Manufactured by Merck Group
Sourced in United States
Resveratrol (3,4,5′-trihydroxy-trans-stilbene) is a naturally occurring polyphenol compound. It is a stilbenoid, a type of natural phenol, and is produced naturally by several plants in response to injury or when the plant is under attack by pathogens, such as bacteria or fungi.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimetylthiazol 2 yl 2 5 diphenyltetrazoliumbromide mtt, by Bio Basic (1 mentions)
Chromatin immunoprecipitation chip assay kit, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Rt premix, by Bioneer (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BioLegend (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcfda, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
L glutamic acid, by Merck Group (1 mentions)
Recombinant human il 1β, by R&D Systems (1 mentions)
Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gapdh, by Abcam (1 mentions)
Sodium tripolyphosphate tpp, by Merck Group (1 mentions)
Recombinant human tnf α, by R&D Systems (1 mentions)
Sodium 13c formate, by Cambridge Isotopes (1 mentions)
7 protocols using resveratrol 3 4 5 trihydroxy trans stilbene
1
Resveratrol Cytotoxicity and Oxidative Stress
2
Chromium(VI) Reduction by Resveratrol
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Cr(VI) [CrO3, purity grade 99.9%; CAS 1333-82-0], 3,4′,5-trihydroxy-trans-stilbene [resveratrol, purity grade ≥ 98%; CAS 501-36-0], AO [CAS 10127-02-3], and EB [CAS 1239-45-8] were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
3
Modulation of MAPK Signaling by Resveratrol
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Human recombinant IL-1β (1 × 109 IU/mg protein) was purchased from R & D Systems (Jiangsu, China). The cytokine was prepared as the previous study [4 (link)]. Medium, fetal bovine serum and penicillin-streptomycin solution were from Gibco-BRL/Thermo Fisher (Co., Ltd., USA); resveratrol (3,4,5′-trihydroxy-trans-stilbene) and dimethyl sulfoxide (DMSO) were provided by Sigma-Aldrich Inc. (St. Louis, MO, USA). U0126, SP600125, and SB203580 from Selleck. cn (Shanghai, China) were dissolved in 0.1% DMSO, which was used as a solvent control. Primary antibodies including Sirt1 and mitogen-activated protein kinases (MAPKs) proteins such as p-ERK, JNK, and p38 MAPK were obtained from CST (Shanghai, China), t-ERK/JNK/p38 MAPK from Proteintech (Wuhan, China), GFAP from Millipore (MA, USA), and GAPDH from Abcam (Shanghai, China). The secondary antibody for Western blot was HRP-conjugated anti-rabbit lgG from Zhongshan Company (Shanghai, China). Please contact the author for more detailed data requests.
4
Encapsulation of Resveratrol Using CSO-Casein
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CSO with molecular weight ~20 kDa was supplied by Amicogen Co. (Jinju, South
Korea). A2 β-casein was provided by Sahmyook University. Resveratrol
(3,4’,5-trihydroxy-trans-stilbene) and sodium tripolyphosphate (TPP) was
obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were
acquired from Sigma-Aldrich (St. Louis, MO, USA).
Korea). A2 β-casein was provided by Sahmyook University. Resveratrol
(3,4’,5-trihydroxy-trans-stilbene) and sodium tripolyphosphate (TPP) was
obtained from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were
acquired from Sigma-Aldrich (St. Louis, MO, USA).
5
Resveratrol and Nitric Oxide Effects
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Materials and reagents were purchased and used as indicated above, from Sigma‐Aldrich (Gillingham, UK), unless otherwise stated, and including molecular Ambion reagents (ThermoFisher Scientific, Hemel Hempstead, UK). The botanical nutrient resveratrol (3,4′,5‐trihydroxy‐transstilbene) was purchased from Sigma‐Aldrich and nitric oxide donors NOC22 (Spermine NONOate, #567703) and SNP (sodium nitroprusside dihydrate, #567538) were purchased from Millipore/Calbiochem (Watford, UK).
6
Immunoblot Analysis of Lipid Metabolism Markers
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Immunoblot analysis of E-FABP was performed using a mouse anti-human E-FABP monoclonal antibody (Mab E-FABP, #sc-365236; Santa Cruz Biotechnology, Santa Cruz, CA, USA) as the primary antibody. Rabbit anti-human p62 polyclonal antibody (Pab p62, #PM045Y), rabbit anti-human Beclin1 polyclonal antibody (Pab Beclin1, #PD017Y), and mouse anti-human LC3 monoclonal antibody (Mab LC3, #M186-3Y) were procured from Medical and Biological Laboratories (Nagoya, Japan). Rabbit anti-human sterol regulatory element-binding protein 1 monoclonal antibody (Mab SREBP1, #NB600-582) was procured from Novus Biologicals (Centennial, CO, USA). Mouse anti-human fatty acid synthase (FAS) monoclonal antibody (Mab FAS, #10038) was procured from Immuno-Biological Laboratories Co., Ltd. (Fujioka, Japan). The rabbit anti-human β-actin monoclonal antibody (Mab β-actin, #4970) was obtained from Cell Signaling Technology (Tokyo, Japan). Resveratrol (3, 4′, 5-trihydroxy-trans-stilbene) and 3-methyladenine (3-MA; PI3K class III inhibitor) were procured from Sigma-Aldrich (St. Louis, MO, USA). Recombinant human TNF-α (R&D Systems, Inc., Minneapolis, MN, USA) was used to stimulate the Ca9-22 cells. For in situ hybridization, probes against human SREBP1 (ACD# 469871), human p62/SQSTM1 (ACD# 415881), and human E-FABP (ACD# 566111-C3) were procured from Advanced Cell Diagnostics Inc. (ACD; Newark, CA, USA).
7
Guinea Pig Dietary Metabolite Supplementation
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Guinea pigs (Dunkin-Hartley), weighing 400-800 g, were fed ad libitum on standard Guinea pig/rabbit pellets, with fresh cabbage leaves and lucerne hay roughage. Animals were maintained on a 12 h light/dark cycle. All experiments were conducted in accordance with the guidelines of the National Health and Medical Research Council of Australia and were approved by the institutional (UNSW) Animal Care Ethics Committee.
Sodium [3-13 C]pyruvate and sodium [ 13 C]formate were purchased from Cambridge Isotope Laboratories Inc (Andover, MA). CACA (cis-4-aminocrotonic acid) and resveratrol (3,4′,5trihydroxy-trans-stilbene) were purchased from Sigma Aldrich (St Louis, MO) and TPMPA (1,5,6-tetrahydropyridin-4-yl)methylphosphinic acid) were purchased from Tocris Cookson, (UK). (±)-cis-(3-Aminocyclopentyl) (butyl)phosphinic acid (cis-ACPBPA) was synthesised as published previously (Hanrahan et al., 2006) , as was cis-2-(aminomethyl)-1carboxycyclopropane (±)-CAMP (Duke et al., 1998) and S-and R-4-amino-1-cyclopent-1-en-1-yl(butyl)phosphinic acids, (S-and R-4-ACPBPA) (Kumar et al., 2008) . All other reagents were of AR grade.
Sodium [3-13 C]pyruvate and sodium [ 13 C]formate were purchased from Cambridge Isotope Laboratories Inc (Andover, MA). CACA (cis-4-aminocrotonic acid) and resveratrol (3,4′,5trihydroxy-trans-stilbene) were purchased from Sigma Aldrich (St Louis, MO) and TPMPA (1,5,6-tetrahydropyridin-4-yl)methylphosphinic acid) were purchased from Tocris Cookson, (UK). (±)-cis-(3-Aminocyclopentyl) (butyl)phosphinic acid (cis-ACPBPA) was synthesised as published previously (Hanrahan et al., 2006) , as was cis-2-(aminomethyl)-1carboxycyclopropane (±)-CAMP (Duke et al., 1998) and S-and R-4-amino-1-cyclopent-1-en-1-yl(butyl)phosphinic acids, (S-and R-4-ACPBPA) (Kumar et al., 2008) . All other reagents were of AR grade.
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