Cells grown on coverslips were fixed in 4% paraformaldehyde and permeabilized in PBS with 0.2% Triton X-100. After washing, cells were blocked in 3% BSA. Primary Mcl-1 (sc819, Santa Cruz), WT1 (NBP2–47858PE, NOVUS) conjugated with PE, pan-Cytokeratin (sc-8018, Santa Cruz) conjugated with PE, Vimentin (sc-66002, Santa Cruz) conjugated with FITC antibodies and TOMM20 (ab209606, Abcam) antibodies conjugated with Alexa Fluor 647 were diluted in blocking solution and incubated overnight at 4°C. DAPI was used as internal control for immunofluorescence. Images were collected with a Zeiss 780 confocal microscope.
Vimentin sc 66002
Manufactured by Santa Cruz Biotechnology
Vimentin (sc-66002) is a primary antibody product offered by Santa Cruz Biotechnology. It is designed to detect vimentin, an intermediate filament protein that is widely expressed in various cell types. The product can be used for applications such as Western blotting, immunohistochemistry, and immunocytochemistry, but a detailed description of its intended use is not available.
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2 protocols using vimentin sc 66002
1
Multi-Marker Immunofluorescence Imaging
2
Multiparametric Immunofluorescence Analysis
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Cells grown on coverslips were fixed in 4% PFA and permeabilized in PBS with 0.2% Triton X-100. After washing, cells were blocked in 3% BSA. Primary Mcl-1 (sc819, Santa Cruz Biotechnology), WT1 (NBP2-47858PE, NOVUS) conjugated with PE, pan-cytokeratin (sc-8018, Santa Cruz Biotechnology) conjugated with PE, Vimentin (sc-66002, Santa Cruz Biotechnology) conjugated with FITC antibodies and TOMM20 (ab209606, Abcam) antibodies conjugated with Alexa Fluor 647 were diluted in blocking solution and incubated overnight at 4°C. DAPI was used as internal control for immunofluorescence. Images were collected with a Zeiss 780 confocal microscope.
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