Recombinant murine TNF-α was purchased from R&D (Minneapolis, MN, USA). Dex was purchased from Sigma-Aldrich (St. Louis, MO, USA). Rg1 was purchased from the National Institutes for Food and Drug Control (Beijing, China). Antibodies against the GR, acetylated lysine, NF-κBp65, phospho-MAPKs, MAPKs, HDAC2, and Nrf2 were purchased from Cell Signaling (Beverly, MA, USA).
Antibodies against the
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against the are affinity-purified reagents designed to detect and study the target protein. They are produced by immunizing animals with the specific antigen and purifying the resulting antibodies. These antibodies can be used to identify, quantify, and study the localization and function of the target protein in various biological samples and experimental systems.
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Lab products found in correlation
2 protocols using antibodies against the
1
Murine TNF-α Modulation by Dex and Rg1
2
Western Blot Analysis of Autophagy Proteins
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Antibodies against ATG5, c-Myc, and FBW7 were purchased from Abcam (USA). Antibodies against the HA tag, the Flag tag, LC3B, and p62 were purchased from Cell Signaling Technology (USA). Antibodies against the HA tag (mouse) and Flag tag (mouse) were purchased from Abmart. Antibodies against ATG5 (mouse), USP28, SKP2, TRIM32, CHIP, and the His tag were purchased from Proteintech (China).
Cells were lysed in RIPA buffer containing 1 mM PMSF. The protein concentration in the supernatant was measured using a BCA Protein Assay Kit. Samples were loaded into 10% or 15% SDS-PAGE gels to resolve proteins. Different amounts of total protein were loaded in each experiment to facilitate the detection of different target proteins. After electrophoresis, the proteins were transferred to PVDF membranes and hybridized with primary antibodies at a dilution of 1:1000. HRP-conjugated secondary antibodies were used at a dilution of 1:5000. An ECL detection system was used to detect the signals on the membranes.
Cells were lysed in RIPA buffer containing 1 mM PMSF. The protein concentration in the supernatant was measured using a BCA Protein Assay Kit. Samples were loaded into 10% or 15% SDS-PAGE gels to resolve proteins. Different amounts of total protein were loaded in each experiment to facilitate the detection of different target proteins. After electrophoresis, the proteins were transferred to PVDF membranes and hybridized with primary antibodies at a dilution of 1:1000. HRP-conjugated secondary antibodies were used at a dilution of 1:5000. An ECL detection system was used to detect the signals on the membranes.
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