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Coronavirus panel plus

Manufactured by NanoString
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The Coronavirus Panel Plus is a multiplex assay that enables the detection and quantification of gene expression for SARS-CoV-2 and related coronavirus targets. It provides comprehensive coverage of viral and host response markers.

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Lab products found in correlation

Facsaria 2 sorp, by BD (1 mentions) Low rna input kit, by NanoString (1 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions)

2 protocols using coronavirus panel plus

1

SARS-CoV-2 Infection of Organoids: FACS and RNA Analysis

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Organoids were infected with SARS-CoV-2 and dissociated and stained for FACS as described above in a BSL3-certified biosafety cabinet. Cells were fixed with 4% PFA for 1 hr at RT to inactivate virus as described above for decontamination out of the BSL3 facility. Cells were sorted for live, single cell, EPCAM+CD45− cells using a BD FACSAria II SORP and RNA was extracted using the protocol described by Russell et. al78 (link). Following RNA extraction, quality was assessed via Agilent Bioanalyzer and cDNA was synthesized using Hs HostReponse LI Primers (Nanostring, XT-HHR-12), Coronavirus Panel Plus (Nanostring, CORONAPP-12) and Low RNA Input Kit (Nanostring, LOW-RNA-48) per manufacturer’s instructions. Following hybridization, transcripts were quantitated using the nCounter® SPRINT Profiler. Samples were run by the LCA Genome Core at University of California, San Francisco.
Choi S.S., van Unen V., Zhang H., Rustagi A., Alwahabi S.A., Santos A.J., Chan J.E., Lam B., Solis D., Mah J., Röltgen K., Trope W., Guh-Siesel A., Lin Z., Beck A., Edwards C., Mallajosyula V., Martin B.A., Dunn J.C., Shrager J., Baric R.A., Pinsky B., Boyd S.D., Blish C.A., Davis M.M, & Kuo C.J. (2023). Organoid modeling of lung-resident immune responses to SARS-CoV-2 infection. Research Square.
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2

Gene Expression Profiling via NanoString Panels

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The autoimmune profiling panel (XT_Hs_AIProfiling_CSO) and coronavirus panel plus, provided by NanoString Technologies (Seattle, WA, USA), were used for gene expression profiling. The autoimmune panel consists of 770 genes, including internal reference genes. The coronavirus panel consists of 10 genes and enables measurement of the SARS‐CoV‐2 virus. For each sample, 300 ng of RNA was hybridised with probes of the panels for 17 h at 65°C using the SimpliAmp Thermal Cycler provided by Applied Biosciences (Waltham, MA, USA). The excess‐unhybridised probes were washed in the PrepStation of the nCounter® FLEX System (GLMX_SCT0002). Gene counts were performed by scanning 490 field‐of‐view using the nCounter digital scanner.
Wismans L.V., Lopuhaä B., de Koning W., Moeniralam H., van Oosterhout M., Ambarus C., Hofman F.N., Kuiken T., Endeman H., Mustafa D.A, & von der Thüsen J.H. (2022). Increase of mast cells in COVID‐19 pneumonia may contribute to pulmonary fibrosis and thrombosis. Histopathology, 82(3), 407-419.
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