Antibody against hsp70

Penicillin-streptomycin was obtained from Lonza (Walkersville, MD, USA). The EZ-Cytox Cell Viability Kit was purchased from Daeil Lab (Seoul, Korea). LA, N-acetyl-l-cysteine (NAC), 2′,7′-dichlorofluorescein diacetate (DCF-DA), ortho-phthaldialdehyde (OPA), chloroform, and trypsin-EDTA solutions were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phosphate-buffered saline (PBS) was purchased from Gibco Life Technologies Inc. (Rockville, MD, USA). Antibody against HSP70 was purchased from Enzo Life Sciences (Aargau, Switzerland). Antibodies against Bcl-2, Bax, phospho-FoxO1, β-actin, anti-rabbit IgG, and anti-mouse IgG were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against SOD1 and MuRF1 were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Vectashield mounting medium containing DAPI was purchased from Vector Laboratories (Burlingame, CA, USA). HPLC-grade methanol and HPTLC silica gel plates were purchased from Merck (Darmstadt, Germany). Ceramide, sphingomyelin, dihydrosphingomyelin, and sphingolipid ceramide N-deacylase (SCDase) were purchased from Avanti Polar Lipid Inc. (Alabaster, AL, USA). All other chemicals were commercially available.

Penicillin-streptomycin was obtained from Lonza (Walkersville, MD, USA). An EZ-Cytox cell viability kit was purchased from Daeil Lab (Seoul, Korea). Antibody against HSP70 was purchased from Enzo Life Sciences, AG (Lausen, Switzerland). SOD1 and β-actin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). N-acetyl-L-cysteine (NAC) and ortho-phthalaldehyde (OPA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sphingomyelin, dihydroSphingomyelin, ceramide, and sphingolipid ceramide N-deacylase (SCDase) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). High Performance Thin Layer Chromatography (HPTLC) silica-gel plate, chloroform, and HPLC grade methanol were purchased from Merck (Darmstadt, Germany). Vectashield mounting medium with DAPI was purchased from Vector Laboratories (Burlingame, CA, USA). All other chemicals used were the highest analytical grade that is commercially available.