Rat total adiponectin acrp30 quantikine elisa kit

Enzymatic colorimetric kits were used to determine serum TC (995280/QCA, Barcelona, Spain), HDL-cholesterol (HDL-c) and VLDL-cholesterol + LDL-cholesterol (VLDL-c + LDL-c) (EHDL-100/Bioassay Systems, California, USA), triglycerides (992330/QCA, Barcelona, Spain), glucose (992330/QCA, Barcelona, Spain) and nonesterified free fatty acids (NEFAs) (WAKO, Neuss, Germany). To analyse the levels of 17β-E2 at baseline and progesterone at the end point, the ELISA E2 kit (CSB-E05110r/CUSABIO, Houston, USA) and the ELISA progesterone kit (CSB-E07282r/CUSABIO Houston, USA) were used, respectively. Circulating insulin levels were measured using a rat/mouse ELISA kit (10-1250-01/MERCODIA, Upssala, Sweden). Serum leptin levels were determined with a rat ELISA kit (EZRL-83K/Millipore, Barcelona, Spain), and serum adiponectin levels were quantified by a Rat Total Adiponectin/ Acrp30 Quantikine ELISA kit (RRP300/R&Dsystems, Minnesota, USA). To quantify the circulating levels of the biomarkers of bone turnover procollagen I amino-terminal propeptide (PINP), osteocalcin (OC) and carboxy-terminal telopeptide of type I collagen (CTX), the PINP ELISA kit (AC-33F1, IDS, UK), the osteocalcin ELISA kit (AC-12F1, IDS, UK) and the CTX (AC-06F1, IDS, UK) were used, respectively.