Cell lysates and protein gel electrophoresis samples were prepared as previously described [11 (link)]. Equal protein amounts of samples were electrophoresed through a reducing SDS polyacrylamide gel and electroblotted onto a nitrocellulose membrane. Membranes were blocked and incubated with antibodies for pRaf-1 (S338; Catalog # 9427), pERK1/2 (T202/Y204; Catalog # 4370), pMEK1/2 (S217/221; Catalog # 9154), pStat3 (Y705; Catalog # 9145), phospho-threonine (Catalog # 9381), ERK1/2, MEK1/2 (Cell Signaling Technology, Danvers, MA, USA), neurotensin receptor (NTR)-1, NTR2, NTR3 and glutaraldehyde-3-phosphate-dehydrogenase (G3PDH) (Santa Cruz Biotechnology). Levels of proteins were detected by using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). Autoradiography was performed using UltraCruz Autoradiography Films (Santa Cruz Biotechnology). The developed films were scanned and optical densities of protein bands were quantified using NIH ImageJ.
Glutaraldehyde 3 phosphate dehydrogenase g3pdh
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Glutaraldehyde-3-phosphate-dehydrogenase (G3PDH) is an enzyme involved in the glycolytic pathway. It catalyzes the conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate, an important step in the breakdown of glucose to produce energy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using glutaraldehyde 3 phosphate dehydrogenase g3pdh
1
Western Blot Analysis of Signaling Proteins
2
Western Blot Analysis of Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RVs were homogenized and protein gel electrophoresis samples were prepared as previously described [21 (link),25 (link)]. Equal protein amounts of samples were electrophoresed through a reducing SDS polyacrylamide gel and electroblotted onto a nitrocellulose membrane. Membranes were blocked and incubated with antibodies for cleaved caspase-3 (Cell Signaling Technology, Inc., Danvers, MA, USA), glutaraldehyde-3-phosphate-dehydrogenase (G3PDH), and collagen A1 (COLA1) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and levels of proteins were detected by using horseradish peroxidase-linked secondary antibodies and an Enhanced Chemiluminescence System (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!