Tumor cells were infected with indicated viruses at MOI = 2. After 24, 48, or 72 h, supernatants of the tumor cell cultures were collected. Mouse serum was collected at indicated times after intratumoral injection of indicated VVs. Serum iPDL1 or GM-CSF was determined using mouse PD-1 DuoSet ELISA kit (R&D, Minneapolis, MN) or mouse GM-CSF ELISA kit (Biolegend, San Diego, CA).
Mouse pd 1 duoset elisa kit
Manufactured by R&D Systems
The Mouse PD-1 DuoSet ELISA kit is a tool used for the quantitative measurement of mouse programmed cell death 1 (PD-1) levels in cell culture supernatants, serum, and plasma samples. The kit provides the necessary components to perform a sandwich enzyme-linked immunosorbent assay (ELISA).
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Lab products found in correlation
2 protocols using mouse pd 1 duoset elisa kit
1
Tumor Cell Virus Infection Assay
2
Detection of rAAV-encoded Proteins
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A double-antibody sandwich ELISA was developed for detection of rAAV-encoded soluble proteins in plasma. A FLAG tag antibody plate (GenScript) was incubated with diluted plasma for 3 h at room temperature to capture the fusion protein with a FLAG tag, followed by washing and detection of p24 by horseradish peroxidase (HRP) anti-p24 antibody (Abcam, ab20365) or sPD1 by anti-PD-1 antibody (R&D Systems, AF1021). Alternatively, the concentration of sPD1 protein in the non-cell fraction after tumor dissociation was determined by mouse PD-1 DuoSet ELISA Kit (R&D Systems) according to the manufacturer’s instructions.
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