Sc 166960

Antibodies against Irgb6 (TGTP; sc-11079), GBP1-5 (sc-166960), Irgm1 (LRG-47; sc-11075), Irgm2 (GTPI; sc-11088), Irgm3 (IGTP; sc-136317), and His-probe (H-3; sc-8036) were purchased from Santa Cruz Biotechnology, Inc. Antibodies against FLAG M2 (F3165) and β-actin (A1978) were purchased from Sigma-Aldrich. Mouse monoclonal and rabbit polyclonal anti-HA antibodies were obtained from BioLegend and Sigma-Aldrich, respectively. Rabbit polyclonal anti-GBP2 and mouse monoclonal anti-p62 (PM045) antibodies were obtained from Proteintech and MBL International, respectively. Anti-ubiquitin mouse monoclonal antibody (FK2; MFK-004) was obtained from Nippon Biotest Laboratories. Mouse monoclonal anti-Irga6 (10D7) and rabbit polyclonal anti-Irgb10 antibodies were provided by Dr JC Howard (Instituto Gulbenkian de Ciência). Rabbit polyclonal anti-GBP1 antibody was provided by Dr EM Frickel (Francis Crick Institute). Rabbit polyclonal anti-GRA7 antibody was provided by Dr JC Boothroyd (Stanford University School of Medicine). Mouse monoclonal anti-GRA2 and rabbit polyclonal anti-Gap45 antibodies were provided by Dr D Soldati-Favre (University of Geneva). Anti-PIP2 antibody (2C11) was purchased from Abcam. Anti-PS antibody (1H6) was purchased from Millipore. Recombinant mouse IFN-γ was purchased from PeproTech.

Antibodies against Gbp2 (ab203238), GFAP (ab53554, ab7260), LCN2 (ab63929), LRP1 (ab92544), MAG (ab89780), MBP (ab40390), NF200 (ab7795), PLP (ab28486) and S100A10 (ab76472) were purchased from Abcam, UK; antibody against CD31 (550274) was purchased from BD Biosciences, USA; antibodies against GFAP (3670S), GFP (2955S, 2956S), p38 (9212S), pp38 (4511S) and β-actin (8457S) were purchased from Cell Signaling Technology, USA; antibodies against CC1 (OP80), dMBP (AB5864) and Olig2 (AB9610) were purchased from Millipore, USA; antibodies against C3d (AF2655), Galectin3 (AF1197), LAMP1 (AF4320) and LCN2 (AF1857) were purchased from R&D Systems, USA; antibodies against Gbp2 (sc-166960) and LRP1 (sc-57351) were purchased from Santa Cruz Biotechnology, USA; antibody against pLRP1 (PA5-101013) was purchased from Thermo Fisher, USA; antibody against Iba1 (019-19741) was purchased from Wako, Japan. All antibodies were used at a dilution of 1:50 to 1:2000 for immunofluorescence and 1:800 to 1:5000 for immunoblotting according to the manufacturer’s instructions. Alexa Fluor 488, 594 and 647 conjugated secondary antibodies were purchased from Jackson, USA.