Sf9 cells (Invitrogen, Carlsbad, CA, United States) were cultured at 27°C in Grace’s insect medium (Invitrogen) supplemented with 10% (v/v) fetal bovine serum (Gibco, Grand Island, NY, United States) and 0.1% (v/v) antibiotic-antimycotic solution (Invitrogen). The recombinant bacmids were generated from bMON14272 (Invitrogen) and maintained in Escherichia coli (E. coli) strain DH10B (Invitrogen), which also contained helper plasmids for homologous recombination and transposition. Spodoptera exigua (S. exigua) larvae were reared on an artificial diet at 28°C (Shorey and Hale, 1965 ).
The polyclonal antiserum of anti-AC75 was prepared in rabbits according to previously published methods (Li et al., 2018 ). The anti-POLH polyclonal antiserum to detect the polyhedrin protein was preserved in the laboratory. Mouse monoclonal anti-actin antibody, horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody and HRP-conjugated goat anti-rabbit antibody were purchased from Proteintech (Wuhan, China).
The polyclonal antiserum of anti-AC75 was prepared in rabbits according to previously published methods (Li et al., 2018 ). The anti-POLH polyclonal antiserum to detect the polyhedrin protein was preserved in the laboratory. Mouse monoclonal anti-actin antibody, horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody and HRP-conjugated goat anti-rabbit antibody were purchased from Proteintech (Wuhan, China).