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Cd20 l26

Manufactured by Roche
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Sourced in Denmark

The CD20 (L26) is a laboratory reagent used in immunohistochemistry (IHC) applications. It is a monoclonal antibody that specifically binds to the CD20 antigen expressed on the surface of B-lymphocytes. The CD20 (L26) reagent can be used to identify and enumerate B-cells in various biological samples.

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Lab products found in correlation

Cd8 c8 144b, by Agilent Technologies (3 mentions) Cd3 2gv6, by Roche (3 mentions) Ventana discovery ultra platform, by Roche (2 mentions) Cd20 clone l26, by Roche (1 mentions) Vectra polaris system, by PerkinElmer (1 mentions) Omnimap anti ms hrp, by Roche (1 mentions) Optiview dab detection kit, by Roche (1 mentions) Ventana benchmark ultra system, by Roche (1 mentions) Opal 570, by Akoya Biosciences (1 mentions) Spectral dapi, by Akoya Biosciences (1 mentions) Opal 520, by Akoya Biosciences (1 mentions) Opal 620, by Akoya Biosciences (1 mentions) Opal 690, by Akoya Biosciences (1 mentions) Cd4 4b12, by Leica (1 mentions) Opal 480, by Akoya Biosciences (1 mentions) Inform advanced image analysis software inform 2.4.1, by Akoya Biosciences (1 mentions) Cd79a sp18, by Roche (1 mentions) Pax 5 sp34, by Roche (1 mentions) Cd138 b 838, by Roche (1 mentions) Mum 1 mrq 43, by Roche (1 mentions)

4 protocols using cd20 l26

1

Multi-marker Immunohistochemistry Profiling

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All stainings were carried out on 3 micrometers paraffin slides using a Ventana Discovery Ultra platform (Ventana, Roche Diagnostics). Double immunohistochemistry was performed on all cases with i) CD3 (2GV6, Ventana) combined to CD20 (L26, Ventana) and ii) CD8 (C8/144B, Dako) combined to PD-L1 (QR1, Diagomics). Stainings were performed with the protocol RUO discovery universal according to the manufacturer’s recommendations with the detection kits OmniMap anti-Rb HRP (760-4311, Ventana) and OmniMap anti-Ms HRP (760-4310, Ventana). The slides were scanned using the PerkinElmer Vectra Polaris System. The double CD20-CD23 immunohistochemistry staining was performed with the primary antibodies CD20 (clone L26, Ventana) and CD23 (clone 1B12, Novocastra). The procedure was performed with a Ventana Benchmark Ultra system (Ventana, Roche Diagnostics) using the Ultraview AP RED and Optiview DAB detection kits (Ventana) following the manufacturer’s recommendations (double stain oDAB-uRED v5 protocol, Ventana).
Vanhersecke L., Brunet M., Guégan J.P., Rey C., Bougouin A., Cousin S., Moulec S.L., Besse B., Loriot Y., Larroquette M., Soubeyran I., Toulmonde M., Roubaud G., Pernot S., Cabart M., Chomy F., Lefevre C., Bourcier K., Kind M., Giglioli I., Sautès-Fridman C., Velasco V., Courgeon F., Oflazoglu E., Savina A., Marabelle A., Soria J.C., Bellera C., Sofeu C., Bessede A., Fridman W.H., Loarer F.L, & Italiano A. (2021). Mature tertiary lymphoid structures predict immune checkpoint inhibitor efficacy in solid tumors independently of PD-L1 expression. Nature cancer, 2(8), 794-802.
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2

Multiplex Immunofluorescence Analysis of TLS

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Multiplexed immunohistofluorescence was performed on TLS-positive cases (identified with HES and CD3/CD20 stainings) using the following antibodies CD20 (L26, Ventana), CD23 (SP23, Ventana), CD21 (2G9, Ventana), CD4 (4B12, Novocastra), CD8 (C8/144B, Dako). Bound primary antibodies were detected using OmniMap anti-Rb HRP (760-4311, Ventana) and OmniMap anti-Ms HRP (760-4310, Ventana) detection kits followed by TSA opal fluorophores (Opal 480, Opal 520, Opal 570, Opal 620 and Opal 690, Akoya Bioscience). The slides were counterstained with spectral DAPI (Akoya Bioscience) and cover-slipped. The slides were scanned using the PerkinElmer Vectra Polaris System, and the multispectral images obtained were unmixed using spectral libraries that were previously built from images stained for each fluorophore (monoplex), using the inForm Advanced Image Analysis software (inForm 2.4.1, Akoya Bioscience) combined with Opal detection kit (Akoya Bioscience).
Vanhersecke L., Brunet M., Guégan J.P., Rey C., Bougouin A., Cousin S., Moulec S.L., Besse B., Loriot Y., Larroquette M., Soubeyran I., Toulmonde M., Roubaud G., Pernot S., Cabart M., Chomy F., Lefevre C., Bourcier K., Kind M., Giglioli I., Sautès-Fridman C., Velasco V., Courgeon F., Oflazoglu E., Savina A., Marabelle A., Soria J.C., Bellera C., Sofeu C., Bessede A., Fridman W.H., Loarer F.L, & Italiano A. (2021). Mature tertiary lymphoid structures predict immune checkpoint inhibitor efficacy in solid tumors independently of PD-L1 expression. Nature cancer, 2(8), 794-802.
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3

Comprehensive Immunohistochemical Analysis of FFPE Tissue

Cited 1 time
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Immunohistochemical stains were performed on formalin-fixed, paraffin-embedded (FFPE) tissue sections following routine
procedures on a Ventana Benchmark automated stainer (Roche Ventana Medical Systems, Tucson, AZ), using the antibodies against CD20
(L26, Roche Diagnostics), CD3 (2GV6, Ventana), CD79a (SP18, Roche Diagnostics), PAX-5 (SP34, Roche Diagnostics), LMP1 (CS
1–4, Dako), EBNA2 (PE2, Abcam), (CD138 (B-838, Roche Diagnostics), kappa (rabbit, Roche Diagnostics), lambda (rabbit,
Roche Diagnostics), CD38 (SPC32, Vector), MUM-1 (MRQ-43, Roche Diagnostics), Ki-67 (MIB-1, Dako), IgG (rabbit, Dako), IgM (rabbit,
Dako), and IgA (rabbit, Dako). Epstein-Barr virus-encoded small RNA (EBER) in situ hybridization was performed as previously
described 13 (link).
Gong S., Crane G.M., McCall C.M., Xiao W., Ganapathi K.A., Cuka N., Davies-Hill T., Xi L., Raffeld M., Pittaluga S., Duffield A.S, & Jaffe E.S. (2018). Expanding the Spectrum of EBV-positive Marginal Zone Lymphomas-A Lesion Associated with Diverse Immunodeficiency Settings. The American journal of surgical pathology, 42(10), 1306-1316.
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4

Multiplex Immunohistochemistry Protocol

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All stainings were conducted on 3.5 μm paraffin slides using a Ventana Discovery Ultra platform (Roche Diagnostics, Rotkreuz, Switzerland). Double immunohistochemistry was carried out on all cases using (i) CD3 (2GV6; Roche Diagnostics) combined with CD20 (L26; Roche Diagnostics) and (ii) CD8 (C8/144B; Dako, Glostrup, Denmark) combined with PD-L1 (QR1; Diagomics, Blagnac, France). Stainings were carried out using the Discovery RUO universal protocol according to the manufacturer's recommendations with OmniMap anti-Rb HRP (760-4311, Roche Diagnostics) and OmniMap anti-Ms HRP (760-4310, Roche Diagnostics) detection kits.
Peyraud F., Guégan J.P., Bodet D., Nafia I., Fontan L., Auzanneau C., Cousin S., Roubaud G., Cabart M., Chomy F., Le Loarer F., Chaput N., Danlos F.X., Planchard D., Even C., Khettab M., Tselikas L., Besse B., Barlesi F., Soria J.C., Marabelle A., Bessede A, & Italiano A. (2022). Circulating L-arginine predicts the survival of cancer patients treated with immune checkpoint inhibitors. Annals of oncology : official journal of the European Society for Medical Oncology, 33(10).
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