Propofol (YZ‐1572503) and D‐sorbitol (S8090) were obtained from Solarbio life science. Sorbitol level assay kits (MAK010) were purchased from Sigma. The SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (9003) was from Cell Signaling Technology. Aldose reductase activity kit (Colorimetric) (ab273276), Antibodies targeting AKR1B1, AKR1B10, phosphorylated NRF2 at Ser40 and NRF2 were purchased from Abcam Company; antibodies targeting α‐tubulin and β‐actin and all unconjugated secondary antibodies were from Santa Cruz Biotechnology; PCNA (Abclonal, A0264), Histone 3 (Abclonal, A2348) and Ki‐67 (Abclonal, A16919) were obtained from Abclonal, and fetal bovine serum (FBS), Alexa‐488‐ and 594‐conjugated secondary antibodies were from Molecular Probes (Invitrogen). NRF2 plasmid was from Public Protein/Plasmid Library. The trizol was purchased from Invitrogen and All‐in‐One First‐Strand cDNA Synthesis Kit and All‐in‐One qPCR Mix were obtained from GeneCopoeia. Subcellular fractionation was conducted using NE‐PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) following the manufacturer's recommendations. All ultrapure reagents were from Promega.
D sorbitol
Manufactured by Solarbio
Sourced in China
D-sorbitol is a sugar alcohol commonly used in laboratory settings. It serves as a polyol, a type of organic compound with multiple hydroxyl groups. D-sorbitol can be utilized in a variety of laboratory procedures and experiments, but a detailed description of its core function is required to maintain an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
All in one qpcr mix, by GeneCopoeia (1 mentions)
Proliferating cell nuclear antigen (pcna), by ABclonal (1 mentions)
Ne per nuclear, by Thermo Fisher Scientific (1 mentions)
Unconjugated secondary antibodies, by Santa Cruz Biotechnology (1 mentions)
Histone 3, by ABclonal (1 mentions)
All in one first strand cdna synthesis kit, by GeneCopoeia (1 mentions)
Zeocin, by Solarbio (1 mentions)
Kanamycin monosulfate, by Solarbio (1 mentions)
Snab 1, by Takara Bio (1 mentions)
Ammonium hydroxide, by Merck Group (1 mentions)
Silver nitrate, by Merck Group (1 mentions)
Sybr premix ex taq, by Takara Bio (1 mentions)
3 protocols using d sorbitol
1
Propofol and Sorbitol Regulation of NRF2
2
Yeast Expression System Protocols
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Restriction enzymes Sac I, SnaB I and Not I were purchased from Takara Bio Inc., Japan, and used as detailed by the manufacturer. Yeast nitrogen base w/o amino acids (YNB), D-sorbitol, Kanamycin monosulfate, Zeocin and geneticin sulfate (G-418S) were obtained from Solarbio, Beijing, China. Oxoid™ peptone and yeast extract were purchased from Thermo Scientific, Germany.
P. pastoris was grown in yeast peptone dextrose medium (YPD, 1% yeast extract, 2% peptone and 2% dextrose) or buffered complex glycerol medium (BMGY, 1% yeast extract, 2% peptone, 100 mM potassium phosphate, pH 6.0, 1.34% yeast nitrogen base, 0.4 mg/mL biotin, 1% glycerol). P. pastoris was induced in buffered complex methanol medium (BMMY, 1% yeast extract, 2% peptone, 100 mM potassium phosphate, pH 6.0, 1.34% yeast nitrogen base, 0.4 mg/mL biotin, 0.5% methanol). YPD plates containing 1 M Sorbitol and 2.5 mg/mL geneticin sulfate were used for selection of positive strains containing the pPIC3.5 K expression vector. E. coli was cultivated in LB medium (0.5% yeast extract, 1% peptone, 1% NaCl). F. graminearum was also grown in YPD medium.
P. pastoris was grown in yeast peptone dextrose medium (YPD, 1% yeast extract, 2% peptone and 2% dextrose) or buffered complex glycerol medium (BMGY, 1% yeast extract, 2% peptone, 100 mM potassium phosphate, pH 6.0, 1.34% yeast nitrogen base, 0.4 mg/mL biotin, 1% glycerol). P. pastoris was induced in buffered complex methanol medium (BMMY, 1% yeast extract, 2% peptone, 100 mM potassium phosphate, pH 6.0, 1.34% yeast nitrogen base, 0.4 mg/mL biotin, 0.5% methanol). YPD plates containing 1 M Sorbitol and 2.5 mg/mL geneticin sulfate were used for selection of positive strains containing the pPIC3.5 K expression vector. E. coli was cultivated in LB medium (0.5% yeast extract, 1% peptone, 1% NaCl). F. graminearum was also grown in YPD medium.
3
Nanoparticle Synthesis and Characterization
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Silver nitrate (AgNO 3 ), tannic acid, and polyvinyl pyrrolidone, and ammonium hydroxide (NH 3 •H 2 O) were purchased from Sigma-Aldrich Chemical Co. d-Sorbitol was purchased from Beijing Solarbio Science and Technology Co. Ltd., and SYBR Premix ExTaq was purchased from Takara Biotech Co. Ltd.
No animals were used in this study, and ethical approval for the use of animals was thus deemed unnecessary.
No animals were used in this study, and ethical approval for the use of animals was thus deemed unnecessary.
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