Quantikine tgfβ1 immunoassay kit

Manufactured by R&D Systems

Sourced in United States

The Quantikine TGFβ1 immunoassay kit is a solid-phase ELISA designed to measure the concentration of total transforming growth factor beta 1 (TGFβ1) in cell culture supernates, serum, and plasma samples. The kit utilizes a quantitative sandwich enzyme immunoassay technique.

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Quantikine kits (80 citations) Quantikine Immunoassay kits (31 citations) Quantikine Immunoassays (15 citations) Quantikines (4 citations)

2 protocols using quantikine tgfβ1 immunoassay kit

Quantifying TGF-β1 Levels in Tissue Samples

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TGF-β1 levels were detected in tissue-culture-conditioned medium (8–16 hr), platelet-rich plasma either by direct assay (active TGFβ1), or following acid treatment to activate latent TGFβ1 (total TGFβ1) with the Quantikine TGFβ1 immunoassay kit (R&D Systems).

Andrade S.S., Sumikawa J.T., Castro E.D., Batista F.P., Paredes-Gamero E., Oliveira L.C., Guerra I.M., Peres G.B., Cavalheiro R.P., Juliano L., Nazário A.P., Facina G., Tsai S.M., Oliva M.L, & Girão M.J. (2017). Interface between breast cancer cells and the tumor microenvironment using platelet-rich plasma to promote tumor angiogenesis - influence of platelets and fibrin bundles on the behavior of breast tumor cells. Oncotarget, 8(10), 16851-16874.

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Quantifying TGF-β1 in MCF-7 Co-cultures

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TGF-β1 levels were detected in MCF-7 cells co-cultured with DMEM (the buffer group), platelets, releasates, or pellets for 40 h with the Quantikine TGF-β1 immunoassay kit (R&D Systems, Minneapolis, MN, US).

Zuo X.X., Yang Y., Zhang Y., Zhang Z.G., Wang X.F, & Shi Y.G. (2019). Platelets promote breast cancer cell MCF-7 metastasis by direct interaction: surface integrin α2β1-contacting-mediated activation of Wnt-β-catenin pathway. Cell Communication and Signaling : CCS, 17, 142.

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