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Mrc 1000 scan head

Manufactured by Nikon
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Sourced in United States

The Nikon MRC 1000 scan head is a high-performance laser scanning confocal microscope component. It features a compact design and is capable of fast scanning for imaging applications.

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Lab products found in correlation

Alexa fluor 488 conjugated wheat germ agglutinin, by Merck Group (2 mentions) Autofluorescence quenching kit, by Vector Laboratories (1 mentions) Optishot, by Nikon (1 mentions) Antifade reagent, by Thermo Fisher Scientific (1 mentions) Antifade, by Thermo Fisher Scientific (1 mentions)

3 protocols using mrc 1000 scan head

1

Quantifying Glycosylation in PAH Biopsies

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Endomyocardial biopsy samples were collected from PAH patients as previously described [47 (link)], and all patients signed a consent form to be in the study. Deparaffinized and rehydrated sections were incubated with fluorescein labeled succinylated wheat germ agglutinin (Vector Laboratories, Burlingame, CA, USA) (1:50) and Alexa 633-WGA overnight at 4 °C, washed in PBS twice, treated with autofluorescence quenching kit (Vector Laboratories), and mounted in Antifade media containing DAPI (Vector Laboratories). Confocal images were collected using a Bio-Rad (Hercules, CA, USA) MRC 1000 scan head mounted on an upright Nikon Optishot (Tokyo, Japan) microscope at the University of Minnesota Imaging Center. FIJI (National Institutes of Health, Bethesda, MD, USA) was used to quantify total intracellular succinylated WGA signal normalized to area.
Prisco S.Z., Rose L., Potus F., Tian L., Wu D., Hartweck L., Al-Qazazi R., Neuber-Hess M., Eklund M., Hsu S., Thenappan T., Archer S.L, & Prins K.W. (2020). Excess Protein O-GlcNAcylation Links Metabolic Derangements to Right Ventricular Dysfunction in Pulmonary Arterial Hypertension. International Journal of Molecular Sciences, 21(19), 7278.
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2

Quantifying Cardiomyocyte T-tubule Structure

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Primary cardiomyocytes and 10‐μm RV and LV cryosections were fixed with 4% paraformaldehyde for 10 minutes at 37°C, washed twice for 5 minutes with PBS, incubated with Alexa Fluor‐488 conjugated wheat germ agglutinin (Sigma‐Aldrich) for 10 minutes at room temperature, and then washed in PBS twice for 5 minutes and mounted in Anti‐Fade Reagent (Molecular Probes). Images were collected using a Bio‐Rad (Hercules, CA) MRC 1000 scan head mounted on an upright Nikon Optishot (Tokyo, Japan) microscope at the University of Minnesota Imaging Center. Z‐stacks were collected and converted into a Z‐projection using ImageJ (National Institutes of Health, Bethesda, MD). T‐tubule structure and regularity were quantified using the TTPower plugin on ImageJ, which uses fast Fourier transformation to quantitate the regularity of t‐tubules as previously described.16, 28
Prins K.W., Tian L., Wu D., Thenappan T., Metzger J.M, & Archer S.L. (2017). Colchicine Depolymerizes Microtubules, Increases Junctophilin‐2, and Improves Right Ventricular Function in Experimental Pulmonary Arterial Hypertension. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(6), e006195.
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3

Quantifying Cardiac Myocyte T-Tubules

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Freshly isolated cardiac myocytes were fixed in 4% paraformaldehyde for 10 min at 37°C, washed with phosphate-buffered saline (PBS) 2 times for 5 min, incubated with AlexaFluor 488 conjugated Wheat Germ Agglutinin (Sigma) for 10 min at room temperature, and then washed in PBS 2 times for 5 min. Cells were mounted in Antifade (Molecular Probes) and imaged on Bio-Rad MRC 1000 scan head mounted on an upright Nikon Optishot microscope at the University Imaging Centers at the University of Minnesota. Z-stacks were collected and converted into a z-projection using ImageJ. T-tubule quantification was performed using the TTPower plugin on ImageJ as described (7 (link)).
Prins K.W., Asp M.L., Zhang H., Wang W, & Metzger J.M. (2016). Microtubule-Mediated Misregulation of Junctophilin-2 Underlies T-Tubule Disruptions and Calcium Mishandling in mdx Mice. JACC: Basic to Translational Science, 1(3), 122-130.
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