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Mouse il 10 elisa max kit

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The Mouse IL-10 ELISA MAX kit is a laboratory equipment product that measures the concentration of interleukin-10 (IL-10) in mouse samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to quantify the target analyte.

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Lab products found in correlation

Lipopolysaccharides (lps), by Merck Group (2 mentions) Goat anti mouse igg igm h l, by Jackson ImmunoResearch (1 mentions) Anti cd40 hm40 3, by BD (1 mentions)

Close spelling variants of this product

IL-10 (167 citations) ELISA MAX kits (48 citations) Mouse ELISA kits (37 citations) ELISAs (15 citations) Mouse IL-10 (4 citations)

2 protocols using mouse il 10 elisa max kit

1

NOD Mouse B-Cell IL-10 Regulation

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Sort-purified splenic B-lymphocytes isolated from NOD mice treated with BAFFR-Fc or ctrl mAb were stimulated with 10 μg/ml LPS (Sigma-Aldrich) or 10 μg/ml goat anti-mouse IgG + IgM (H+L) (Jackson ImmunoResearch) plus 1 μg/ml anti-CD40 (HM40-3, BD Biosciences) for 3 days. In another experiment, TACIhigh or TACIlow B-lymphocytes isolated from NOD mice were sorted and stimulated with 10 μg/ml LPS (Sigma-Aldrich) for 3 days. IL-10 concentration in culture supernatants was determined using the Mouse IL-10 ELISA MAX kit (Biolegend) according to the manufacturer’s recommended protocol.
Wang Q., Racine J.J., Ratiu J.J., Wang S., Ettinger R., Wasserfall C., Atkinson M.A, & Serreze D.V. (2017). Transient BAFF blockade inhibits type 1 diabetes development in NOD mice by enriching immunoregulatory B-lymphocytes sensitive to deletion by anti-CD20 co-therapy,. Journal of immunology (Baltimore, Md. : 1950), 199(11), 3757-3770.
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2

IL-10 Secretion by CD73+ B-Cells

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CD73+ or CD73- B-lymphocytes were sorted as described above and cultured at 5.0×105 cells/ mL in XVIVO-20 medium with 0 or 10 μg/mL LPS (Sigma-Aldrich) for 3 days. IL-10 concentration in culture supernatants was determined using the Mouse IL-10 ELISA MAX kit (BioLegend).
Ratiu J.J., Racine J.J., Hasham M.G., Wang Q., Branca J.A., Chapman H.D., Zhu J., Donghia N., Philip V., Schott W.H., Wasserfall C., Atkinson M.A., Mills K.D., Leeth C.M, & Serreze D.V. (2017). Genetic and small molecule disruption of the AID/RAD51 axis similarly protect NOD mice from type 1 diabetes through expansion of regulatory B-lymphocytes,. Journal of immunology (Baltimore, Md. : 1950), 198(11), 4255-4267.
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