Ginsenoside Rh3 ([HPLC] ≥ 98%) was obtained from the Biomedical Research Institute of Northwest University (Xi’an, Shaanxi, China—lot number: 20,210,723; validity period extending until July 2023). RPMI 1640 was purchased from HyClone (Logan, UT, USA). Methylthiazolyldiphenyl tetrazolium bromide (MTT) and cobalt chloride (CoCl2) were purchased from Beyotime Biotechnology (Shanghai, China). Trypsin, penicillin–streptomycin (pen–strep), and crystal violet were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Fetal bovine serum (FBS) was purchased from Biological Industries (Cromwell, CT, USA). U0126 was purchased from MedChemExpress. Bouin’s solution was purchased from Phygene (Fuzhou, Fujian, China). The list of antibodies used is shown in Supplementary Table S1 .
Bouin s solution
Manufactured by Phygene
Sourced in China
Bouin's solution is a fixative used in histological specimen preparation. It is a mixture of picric acid, formaldehyde, and acetic acid. Bouin's solution is used to preserve and harden tissues, allowing for thin sectioning and staining prior to microscopic examination.
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Lab products found in correlation
2 protocols using bouin s solution
1
Ginsenoside Rh3 Cellular Effects
2
Comprehensive Histological and Ultrastructural Analysis of Rat Liver and Testis
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The liver and testis were removed, subjected to necroscopic analysis, and weighted to determine the wet weight of each organ. The relative organ weight was then calculated for each organ (organ weight/body weight).
Next, the left testis of 9 rats in each group were randomly selected to fix in 10% neutral formalin, and then the organs were embedded in paraffin and sliced into 4 µm thick sections. Finally, these sections were stained with HE and examined under the light microscope.
In addition, part of the right testes of the rats was taken and fixed in Bouin’s solution (Phygene, China) for 18 hours. Then, the Bouins solution was replaced with 10% neutral formalin and the tissues were fixed twice for 24 hours each time. Finally, the organs were stained with Schiff and hematoxylin, the cross-section of the seminiferous tubules was observed and photographed under an oil microscope.
The other part of right testes was fixed in 10% glutaric acid (pH=7.2–7.4), and post-fixed in osmium tetroxide. Then, ethanol and acetone were used for gradient dehydration. The samples were embedded in epoxy resin and hardened at room temperature for 16 hours. Subsequently, sections were stained with uranyl acetate and lead nitrate. Finally, testis's ultrastructure was observed and pictures were taken with a TEM.
Next, the left testis of 9 rats in each group were randomly selected to fix in 10% neutral formalin, and then the organs were embedded in paraffin and sliced into 4 µm thick sections. Finally, these sections were stained with HE and examined under the light microscope.
In addition, part of the right testes of the rats was taken and fixed in Bouin’s solution (Phygene, China) for 18 hours. Then, the Bouins solution was replaced with 10% neutral formalin and the tissues were fixed twice for 24 hours each time. Finally, the organs were stained with Schiff and hematoxylin, the cross-section of the seminiferous tubules was observed and photographed under an oil microscope.
The other part of right testes was fixed in 10% glutaric acid (pH=7.2–7.4), and post-fixed in osmium tetroxide. Then, ethanol and acetone were used for gradient dehydration. The samples were embedded in epoxy resin and hardened at room temperature for 16 hours. Subsequently, sections were stained with uranyl acetate and lead nitrate. Finally, testis's ultrastructure was observed and pictures were taken with a TEM.
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