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Polyclonal glial fibrillary acidic protein

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Polyclonal glial fibrillary acidic protein (GFAP) is a protein that is primarily found in the astrocytes of the central nervous system. It is a commonly used marker for the identification and characterization of astrocytes in various research and diagnostic applications.

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3 protocols using polyclonal glial fibrillary acidic protein

1

Immunohistochemical Labeling of Retinal Cells

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Polyclonal Vps34 antibody was obtained from Cell Signaling (Danvers, MA, USA). Polyclonal antisera to the p85α regulatory subunit of class I PI3K were obtained from Upstate Biotechnology, Inc. (Lake Placid, NY, USA). The goat secondary antibody was procured from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-red/green cone opsin (M-opsin), anti-cone arrestin, anti-S-opsin, and rabbit and mouse secondary antibodies were obtained from Millipore (Billerica, MA, USA). Mouse monoclonal anti-Cre antibody suitable for immunohistochemistry was purchased from Abcam (Cambridge, MA, USA). Monoclonal 1D4 rhodopsin antibody was a kind gift from Dr. James F. McGinnis (University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA). Peanut agglutinin (PNA) and secondary antibodies were purchased from Vector Laboratories (Burlingame, CA, USA). 4′,6-diamidino-2-phenylindole (DAPI) used for nuclear staining was procured from Invitrogen Molecular Probes (Carlsbad, CA, USA). The monoclonal anti-arrestin antibody was a kind gift from Dr. Paul Hargrave (University of Florida, Gainesville, FL, USA). The monoclonal glutamine synthetase (GS) antibody was purchased from Abcam (Cambridge, MA, USA). Polyclonal glial fibrillary acidic protein (GFAP) was purchased from Dako (Carpinteria, CA, USA).
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2

Immunohistochemical Analysis of Retinal Proteins

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Polyclonal IGF-1R antibody, mouse monoclonal Cre antibody, and mouse glutamine synthetase antibodies suitable for immunohistochemistry were purchased from Abcam (Cambridge, MA). Rabbit polyclonal IGF-1R antibody was purchased from Protientech (Rosemount, IL). Rabbit polyclonal red/green cone opsin (M-opsin), cone arrestin, actin, and rabbit and mouse secondary antibodies were obtained from Millipore (Billerica, MA). Monoclonal 1D4 rhodopsin antibody was a kind gift from Dr. James F. McGinnis (University of Oklahoma Health Sciences Center). DAPI used for nuclear staining was procured from Invitrogen-Molecular Probes (Carlsbad, CA). The monoclonal anti-arrestin antibody was a kind gift from Dr. Paul Hargrave (University of Florida, Gainesville). Polyclonal glial fibrillary acidic protein (GFAP) was purchased from Dako (Carpinteria, CA).
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3

Immunohistochemical Antibody Characterization

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Polyclonal LDHA, LDHB, and aldolase C antibodies were purchased from Proteintech (Rosemount, IL). Rabbit polyclonal red/green cone opsin (M-opsin), S-opsin, cone arrestin, actin, and rabbit and mouse secondary antibodies were obtained from Millipore (Billerica, MA). Monoclonal 1D4 rhodopsin antibody was a kind gift from Dr. James F. McGinnis (University of Oklahoma Health Sciences Center). DAPI used for nuclear staining was procured from Invitrogen-Molecular Probes (Carlsbad, CA). Polyclonal pPKM2 (Y105), PKM2, PKM1, PDH, hexokinase 1, and hexokinase 2 antibodies were obtained from Cell Signaling (Danvers, MA). The monoclonal anti-arrestin antibody was a kind gift from Dr. Paul Hargrave (University of Florida, Gainesville). Polyclonal glial fibrillary acidic protein (GFAP) was purchased from Dako (Carpinteria, CA). Monoclonal GS antibody was purchased from Abcam (Cambridge, MA). The monoclonal Pde6β antibody was purchased from Santa Cruz Biotechnology (Dallas, TX).
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