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Dc sign apc

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DC-SIGN-APC is a recombinant protein that binds to the DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin) receptor. DC-SIGN is a C-type lectin receptor expressed on the surface of dendritic cells and plays a role in pathogen recognition and immune cell interaction. The DC-SIGN-APC protein is conjugated with the fluorescent dye allophycocyanin (APC), which allows for detection and analysis of DC-SIGN-expressing cells using flow cytometry or other fluorescence-based techniques.

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Cd11c apc, by BD (2 mentions) Cd83 pe, by Thermo Fisher Scientific (2 mentions) Cd163 apc, by BioLegend (2 mentions) Cd3 apc, by Miltenyi Biotec (2 mentions) Hla dr fitc, by Miltenyi Biotec (2 mentions) Zombie dye yellow staining, by BioLegend (2 mentions) Cd45 vioblue450, by Cytek Biosciences (2 mentions) Cd3 viogreen, by Miltenyi Biotec (2 mentions) Cd103 pe cy7, by Thermo Fisher Scientific (2 mentions) Cd11c percp cy5, by BioLegend (2 mentions) Dc sign pe, by R&D Systems (2 mentions) Ccr7 pe cy7, by Miltenyi Biotec (2 mentions) Cd1a af700, by BioLegend (2 mentions) Cd1a fitc, by Thermo Fisher Scientific (2 mentions) Cd86 e710, by Thermo Fisher Scientific (2 mentions) Ccr5 pe cy7, by BD (2 mentions) Cd207 apc, by BioLegend (2 mentions) Dc sign fitc, by R&D Systems (2 mentions) Cd11b pe, by Cytek Biosciences (2 mentions) Cd1c pe dazzle, by BioLegend (2 mentions)

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DC-SIGN (11 citations)

2 protocols using dc sign apc

1

Multicolor Flow Cytometry Analysis

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Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD11c-APC, CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR7-PE-Cy7, HLA-DR-FITC, CD3-VioGreen (Miltenyi Biotec), CD3-APC, CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, CD163-APC, HLA-DR-BV570, CD207-APC, CD1a AF700 (Biolegend), CD103-PE-Cy7, CD83-PE, CD14-e780, CD1a-FITC, CD86-e710 (eBiosciences, San Diego, CA), DC-SIGN-FITC, DC-SIGN-PE, DC-SIGN-APC (R&D systems, Minneapolis, MN). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. The gating strategy is shown in supplementary Figure 1.
Rodriguez-Garcia M., Shen Z., Barr F.D., Boesch A.W., Ackerman M.E., Kappes J.C., Ochsenbauer C, & Wira C.R. (2016). Dendritic Cells from the Human Female Reproductive Tract Rapidly Capture and respond to HIV. Mucosal immunology, 10(2), 531-544.
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2

Multicolor Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mixed cell suspensions were stained for surface markers with combinations of the following antibodies: CD45-vioblue450, CD11b-PE (Tonbo, San Diego, CA), CD11c-APC, CCR5-PE-Cy7 (BD Biosciences, San Jose, CA), CCR7-PE-Cy7, HLA-DR-FITC, CD3-VioGreen (Miltenyi Biotec), CD3-APC, CD11c-PerCp-Cy5.5, CD1c-PE-dazzle, CD163-APC, HLA-DR-BV570, CD207-APC, CD1a AF700 (Biolegend), CD103-PE-Cy7, CD83-PE, CD14-e780, CD1a-FITC, CD86-e710 (eBiosciences, San Diego, CA), DC-SIGN-FITC, DC-SIGN-PE, DC-SIGN-APC (R&D systems, Minneapolis, MN). Dead cells were excluded with 7AAD (Southern Biotech) or zombie dye yellow staining (Biolegend). Analysis was performed on 8-color MACSQuant 10 (Miltenyi biotech) or Gallios (Beckman Coulter) flow cytometers and data analyzed with FlowJo software (Tree Star, Inc. Ashland, OR). Expression of surface markers is shown as percentage of positive cells. Fluorescence minus one (FMO) strategy was used to establish appropriate gates. The gating strategy is shown in supplementary Figure 1.
Rodriguez-Garcia M., Shen Z., Barr F.D., Boesch A.W., Ackerman M.E., Kappes J.C., Ochsenbauer C, & Wira C.R. (2016). Dendritic Cells from the Human Female Reproductive Tract Rapidly Capture and respond to HIV. Mucosal immunology, 10(2), 531-544.
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