Gpr41

Manufactured by Merck Group

Gpr41 is a laboratory equipment product designed to facilitate research and analytical processes. It serves as a versatile tool for various applications within the scientific community.

Automatically generated - may contain errors

Lab products found in correlation

Propionate, by Merck Group (1 mentions) Propionic acid, by Merck Group (1 mentions) Sodium propionate, by Merck Group (1 mentions) Dmem f12 medium, by Thermo Fisher Scientific (1 mentions) Glpg0974, by Merck Group (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Abcam (1 mentions) Ripa buffer, by Boston BioProducts (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Gpr43, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

2 protocols using gpr41

Fucose Regulates Gut Microbiome Metabolism

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To address whether fucose affects ISCs by regulating gut microbe-mediated metabolism, ileal contents were used to treat organoids as previously described.^{16 (link)} Briefly, 100 mg of ileal contents was suspended in 1 ml serum-free DMEM/F12 medium (Gibco), vortexed for 1 h at 4°C, centrifuged at 4000 rpm for 10 min, and filtered using a 0.22 μm filter (Millipore). The organoids were treated with 0.01% ileal content. 0.1 μM Gpr41 and 3 mM Gpr43 antagonist β-hydroxybutyrate (Sigma-Aldrich) and GLPG0974 (Sigma-Aldrich) were used to inhibit Gpr41/Gpr43. One millimolar propionic acid (Sigma-Aldrich) and sodium propionate (Sigma-Aldrich) was used to examine the role of propionate metabolism in fucose-induced ISCs promotion. One hundred nanomolar Wnt-c59 was used to inhibit Wnt signaling.

Duan C., Wu J., Wang Z., Tan C., Hou L., Qian W., Han C, & Hou X. (2023). Fucose promotes intestinal stem cell-mediated intestinal epithelial development through promoting Akkermansia-related propanoate metabolism. Gut Microbes, 15(1), 2233149.

+ Open protocol

+ Expand

Whole Kidney Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole kidney tissues were homogenized with RIPA buffer (Boston BioProducts, Inc., Ashland, MA) containing protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and phenylmethylsulfonyl fluoride. 100 μg of protein from each sample were loaded and electrophoresed in sodiumdodecyl sulphate-polyacrylamide gel electrophoresis, and transferred onto PVDF membranes. Membranes were probed with appropriate primary antibody at 4°C overnight. Olfr78 (Abcam, ab140907), Gpr41 (Sigma, SAB4501281), Gpr43 (Santa Cruz, sc293202), Tgfβ (Abcam, ab64715), Rorcγ (Abcam, ab207082), Il6 (Abcam, ab6672), Gpr120, (ThermoFisher, PA5-50973), Gapdh (Santa Cruz, sc32233). Respective Horseradish Peroxidase-conjugated secondary antibody incubation was for 2 h at room temperature. Membranes were developed using chemiluminescence (Pierce ECL Western blotting substrate; Thermo Scientific, Rockford, IL). Gapdh was used as loading control and band intensity was quantified using ImageJ software.

Weber G.J., Foster J., Pushpakumar S.B, & Sen U. (2018). Altered microRNA regulation of short chain fatty acid receptors in the hypertensive kidney is normalized with hydrogen sulfide supplementation. Pharmacological research, 134, 157-165.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!