Irdye800 labeled donkey anti mouse

The cell lysates were fractioned by 10% SDS-PAGE and then blotted onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membranes were incubated overnight with primary antibodies at a dilution of 1:1000, with the exception of anti-β-tubulin (1:2000). The antibodies used for immunoblotting were: rabbit anti-NF-κB p50 (sc-7178, Santa Cruz), rabbit NF-κB p65 (sc-7151, Santa Cruz), rabbit anti-Bcl-3 (sc-185, Santa Cruz), rabbit anti-STAT3 (sc-482, Santa Cruz), rabbit anti-IRF2 (ab124744, Abcam), rabbit anti-IκBα (9242S, Cell Signaling Technology, Danvers, MA, USA), mouse anti-phospho-IκBα (5A5, Cell Signaling), mouse anti-β-tubulin (T8328, Sigma-Aldrich, St. Louis, MO, USA), and mouse anti-lamin A+C (ab8984, Abcam). The membranes were then incubated with IRDye700-labeled donkey anti-mouse or anti-rabbit, or IRDye800-labeled donkey anti-mouse (LI-COR Biosciences) at a 1:5000 dilution. The blots were detected using an Odyssey^® Infrared Imaging System (LI-COR Biosciences).

Membranes were incubated overnight with primary antibodies at a dilution of 1:1,000. The antibodies used for immunoblotting include rabbit anti-NF-κB p50 (sc-7178; Santa Cruz), rabbit anti-NF-κB p65 (sc-7151; Santa Cruz), rabbit anti-Bcl-3 (sc-185; Santa Cruz), mouse anti-β-tubulin (sc-55529; Santa-Cruz), rabbit anti-IκBα (9242S; Cell Signaling), mouse anti-phospho-IκBα (5A5; Cell Signaling), mouse anti-lamin A+C (ab8984; Abcam), and mouse anti-LMP1 (OT21C; kindly provided by Jaap Middeldorp, VU University, Amsterdam, The Netherlands). Membranes were then incubated with IRDye 700-labeled donkey anti-mouse or anti-rabbit or IRDye 800-labeled donkey anti-mouse (Li-Cor Biosciences) at a 1:5,000 dilution. Blots were detected using an Odyssey infrared imaging system.