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Bortezomib btz

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Bortezomib (BTZ) is a proteasome inhibitor used in laboratory research. It functions by blocking the activity of the proteasome, a large protein complex responsible for the degradation of unwanted or damaged proteins within cells.

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Lab products found in correlation

Lithium chloride (licl), by Merck Group (1 mentions) Lithium acetate, by Merck Group (1 mentions) Bromocriptine, by Cayman Chemical (1 mentions) Nimesulide, by Cayman Chemical (1 mentions) Mesalamine, by Cayman Chemical (1 mentions) Tws119, by Cayman Chemical (1 mentions) Flt3l, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions) G csf, by Thermo Fisher Scientific (1 mentions) Wee1 d10d2, by Cell Signaling Technology (1 mentions) P27 kip1 d69c12, by Cell Signaling Technology (1 mentions) Hela cells ccl 2, by American Type Culture Collection (1 mentions) Sh sy5y cell, by American Type Culture Collection (1 mentions) Cddo me, by Merck Group (1 mentions) Bafilomycin a1 baf, by Merck Group (1 mentions) Hela cell, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions)

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Bortezomib (129 citations)

5 protocols using bortezomib btz

1

Screening of Small Molecule Library

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The 1040 compounds used for the small molecule screen were from the Prestwick Chemical Library (Illkirch, France). The following compounds were obtained from Cayman Chemical (Ann Arbor, MI): TWS119 (#10011251), ipsapirone (#22075), bromocriptine (#14598), urapidil (#29004), trimipramine (#15921), dichlorphenamide (#23658), nimesulide (#70640), and mesalamine (#70265). Lithium chloride (LiCl) was obtained from Sigma (St. Louis, MO, #62476), lithium acetate was obtained from Aldrich Chemical Company (Milwaukee WI, #21,319–5), and bortezomib (BTZ) was obtained from EMD Millipore (#179324-69-7).
Hope K.A., Berman A.R., Peterson R.T, & Chow C.Y. (2022). An in vivo drug repurposing screen and transcriptional analyses reveals the serotonin pathway and GSK3 as major therapeutic targets for NGLY1 deficiency. PLoS Genetics, 18(6), e1010228.
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2

Bortezomib Cytokine Signaling Regulation

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Bortezomib (BTZ) was purchased from Merck. For the in vitro experiments, a 10 mM stock solution was prepared by dissolving it in dimethyl sulfoxide (DMSO) purchased from Sigma-Aldrich. The concentration of DMSO in the culture was kept from 0.1 to 0.2%. The stock solution was stored at −20 °C until use. The antibodies for the immunoblots, including GAPDH (14C10), P21waf1/cip1 (12D1), P53, P27kip1 (D69C12), CDK1 (POH1), CYCLIN B1 (D5C10), and WEE1 (D10D2), were purchased from Cell Signaling (Beverly, MA, USA), while Anti-DDK(FLAG) (TA50011-100) was purchased from OriGene Technologies, Inc. (Rockville, MD 20850, USA).
Five cytokines—IL-3, IL-6, SCF, G-CSF, and Flt3-L—were purchased from PEPRO Tech (Rocky Hill, NJ, USA).
Din R.U., Jiao A., Qiu Y., Mohan A.A., Yuen K.C., Wong H.T., Wan T.M., Wong P.O, & Sin C.F. (2023). Bortezomib Is Effective in the Treatment of T Lymphoblastic Leukaemia by Inducing DNA Damage, WEE1 Downregulation, and Mitotic Catastrophe. International Journal of Molecular Sciences, 24(19), 14646.
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3

Antiviral Compound Screening for DENV and ZIKV

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All viruses were obtained from Emerging Infections Research Resources Repository (BEI Resources, Manassas, VA, USA). Monkey plasmas neutralizing DENV or ZIKV were provided by Dr. Gregory Gromowski, Viral Diseases Branch, Walter Reed Army Institute of Research. Aedes albopictus mosquito C6/36 cells (ATCC CRL-1660), African green monkey kidney epithelial Vero cells (CCL-81), human embryonic kidney HEK-293 cells (CRL-1573), SH-SY5Y cells (CRL-2266), U-251 MG cells (HBT-17), and HeLa cells (CCL-2) were obtained from ATCC (Manassas, VA, USA). Huh-7 cells were provided by Dr. Hongbing Wang, University of Maryland School of Pharmacy. All cells were cultured in complete Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) as growth medium or 2% FBS as maintenance medium. Bortezomib (BTZ), CDDO-me, and PU-WS13 were purchased from Sigma-Aldrich (St. Louis, MO, USA), Adooq Biosciences (Irvin, CA, USA), EMD Chemicals (San Diego, CA, USA), respectively. The sources for antibodies are: Anti-grp94 antibody (Affinity Bioreagents, Golden, CO, USA), anti-OS9 antibody (Thermo Fisher Scientific, Waltham, MA, USA), Anti-Hsp70 and anti-Tubulin antibodies (Enzo Life Sciences, Farmingdale, NY, USA), anti-Hsp90 and anti-BiP antibodies ( BD Biosciences, San Jose, CA, USA), Anti-DENV2 E protein and anti-DENV2 NS3 protein antibodies (GeneTex, San Antonio, TX, USA).
Rothan H.A., Zhong Y., Sanborn M.A., Teoh T.C., Ruan J., Yusof R., Hang J., Henderson M.J, & Fang S. (2019). Small molecule grp94 inhibitors with antiviral activity against Dengue and Zika virus. Antiviral research, 171, 104590.
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4

Mtb mc^2 6230 Cultures Treated with Bortezomib

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Mtb mc26230 cultures were grown as previously described 66 (link) for 4.5 days to mid-log phase, then treated for 48 hrs with 40μM bortezomib (BTZ, Sigma-Aldrich) dissolved in DMSO 66 (link). DMSO was used as a vehicle control.
Schneider R.F., Hallstrom K., DeMott C, & McDonough K.A. (2024). Conditional protein splicing of the Mycobacterium tuberculosis RecA intein in its native host. bioRxiv.
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5

Analyzing Rhodopsin Degradation in HeLa Cells

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HeLa cells were obtained from American Type Culture Collection (ATCC) and were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Gibco) supplemented with 10% (v/v) FBS and 5% penicillin-streptomycin. Cell lines were maintained at 37°C, 5% CO2 in a humidified incubator according to the guidelines provided by the vendors. To analyze rhodopsin degradation, cells were plated for 24 h and then treated with 200-nM Bafilomycin A1 (Baf) (Sigma–Aldrich, B1793) for 3 h, 100-μg/ml cycloheximide (CHX) (Sigma–Aldrich, C4859), 100-mM Bortezomib (BTZ) (Sigma–Aldrich, 5043140001) and 10 μM of NSC668394 or DMSO as previously reported (Bulut et al., 2012 (link)).
Intartaglia D., Giamundo G., Naso F., Nusco E., Di Giulio S., Salierno F.G., Polishchuk E, & Conte I. (2022). Induction of Autophagy Promotes Clearance of RHOP23H Aggregates and Protects From Retinal Degeneration. Frontiers in Aging Neuroscience, 14, 878958.
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