Anti cle caspase3

Human gastric cancer cell lines SGC-7901, BGC- 823 and KATO III were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The cells were routinely cultured in RPMI 1640 medium (Gibco, Eggenstein, Germany) containing 10% heat-inactivated fetal bovine serum (Hyclone, Logan, UT), 100 units/ml penicillin, and 100 ug/ml streptomycin in a humidified cell incubator with an atmosphere of 5% CO2 at 37°C. FITC Annexin V apoptosis Detection Kit I and propidium iodide (PI) were purchased from BD Pharmingen (Franklin Lakes, NJ). 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) and JC-1 were purchased from Invitrogen (Carlsbad, CA). Antibodies including anti-Cdc2, anti-XBP-1, a'nti-Bcl-2, anti-Bax, anti-Cyclin B1, anti-cleaved PARP, anti-MDM-2, anti-p53, anti-TrxR1, anti-Ki67, anti-GAPDH, goat anti-mouse IgG-HRP, donkey anti-rabbit IgG-HRP and donkey anti-goat IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies including anti-ATF-4, anti-p-EIF2α, anti-CHOP, anti-Cle-caspase3, were purchased from Cell Signaling Technology (Danvers, MA).

Isoalantolactone (Tauto Biotech, Shanghai, China) was suspended in dimethyl sulfoxide and stored at − 20 °C. N-acetyl-L-cysteine (NAC) and catalase were purchased from Sigma (St. Louis, MO, USA). Antibodies including anti-GAPDH, m-IgGκ BP-HRP and mouse anti-rabbit IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies including anti-CHOP, anti-ATF4, anti-p-eIF2α, anti-eIF2α, anti-Bcl-2 and anti-cle-caspase-3 were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies including anti-p-STAT3 and anti-STAT3 were purchased from Abcam (Cambridge, MA, USA). FITC Annexin V apoptosis Detection Kit I and Propidium Iodide (PI) were purchased from BD Pharmingen (Franklin Lakes, NJ, USA). Human prostate cancer cell lines PC-3 and DU145 were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The cells were maintained in RPMI 1640 medium containing 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator with an atmosphere of 5% CO₂.