Ab150068

Manufactured by Abcam

Sourced in Canada

Ab150068 is a primary antibody product manufactured by Abcam. It is designed for use in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab8822, by Abcam (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions) Ab5694, by Abcam (2 mentions) Ab5450, by Abcam (1 mentions) Ab150133, by Abcam (1 mentions) Ab150066, by Abcam (1 mentions)

3 protocols using ab150068

HMGR1S Antibody Production and Characterization

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The catalytic domain of Arabidopsis HMGR1S (CD1) produced in Escherichia coli was used as immunogen to produce a polyclonal antibody in rabbit and the resulting serum was immunosubstracted to remove IgG reacting against the bacterial proteins [48 (link)]. The immunopurified serum (Ab-CD1-i) was used as primary antibody at 1:500 for whole mount and 1:1000 for transmission EM. Anti-rabbit IgG secondary antibodies for HMGR detection were code Ab150066 (Abcam, Toronto, ON, Canada) coupled to Alexa Fluor-555 at 1:1000 for whole mount (Figure 1a,b), code Ab150068 (Abcam, Toronto, ON, Canada) coupled to Alexa Fluor-594 at 1:1000 for whole mount (Figure 1g), code 111-215-144 (Jackson Immunoresearch, Cambridge, UK) coupled to an 18-nm gold particle at 1:30 for transmission EM (Figure 1d) and code 111-205-144 (Jackson Immunoresearch, Cambridge, UK) coupled to a 12-nm gold particle at 1:30 for transmission EM (Figure 1e,f,l,m).
GFP was detected with Ab-5450 (Abcam, Toronto, ON, Canada) as the primary antibody at 1:1000 for whole mount and transmission EM. Anti-goat IgG secondary antibodies for GFP detection were code Ab150133 (Abcam, Toronto, ON, Canada) coupled to Alexa Fluor-488 at 1:1000 for whole mount, and code 705-215-147 (Jackson Immunoresearch, Cambridge, UK) coupled with an 18-nm gold particle at 1:15 for transmission EM.

Grados-Torrez R.E., López-Iglesias C., Ferrer J.C, & Campos N. (2021). Loose Morphology and High Dynamism of OSER Structures Induced by the Membrane Domain of HMG-CoA Reductase. International Journal of Molecular Sciences, 22(17), 9132.

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Quantifying Myocardial Angiogenesis Post-Infarction

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At the 4-week time point after LAD ligation and treatment, the hearts were explanted. Sections were washed 3 times in PBS, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and blocked in 10% fetal bovine serum (Gibco, Gaithersburg, Md) for 1 hour at 37°C. Primary antibodies were diluted 1:150 in PBS and incubated for 2 hours at 37°C. Secondary antibodies were diluted in PBS and incubated for 2 hours at 37°C. Primary antibodies included sheep anti–von Willebrand factor conjugated to fluorescein isothiocyanate (Abcam [Cambridge, Mass], ab8822) and rabbit anti-smooth muscle actin (Abcam, ab5694). Donkey anti-rabbit conjugated to Alexa Fluor 594 (Abcam, ab150068) was used as a secondary antibody (1:200). Nuclei were stained with DAPI (Vector Laboratories, Burlingame, Calif). Vasculature was quantified using ImageJ (National Institutes of Health, Bethesda, Md). Measurements were made 1 high-power field (hpf) from the infarct at 20× magnification.

Gaffey A.C., Chen M.H., Trubelja A., Venkataraman C.M., Chen C.W., Chung J.J., Schultz S., Sehgal C.M., Burdick J.A, & Atluri P. (2018). Delivery of progenitor cells with injectable shear-thinning hydrogel maintains geometry and normalizes strain to stabilize cardiac function after ischemia. The Journal of thoracic and cardiovascular surgery, 157(4), 1479-1490.

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Quantifying Vascular Changes Post-LAD Ligation

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At the 4 week time point following LAD ligation and treatment, the hearts were explanted. Sections were washed 3 times in PBS, fixed in 4% paraformaldehyde for 10 minutes at room temperature, and blocked in 10% fetal bovine serum (Gibco) for 1 hour at 37°C. Primary antibodies were diluted 1:150 in PBS and incubated for 2 hours at 37°C. Secondary antibodies were diluted in PBS and incubated for 2 hours at 37°C. Primary antibodies included sheep anti-von Willebrand factor conjugated to FITC (Abcam, ab8822) and rabbit anti-smooth muscle actin (Abcam, ab5694). Donkey anti-rabbit conjugated to Alexa Fluor 594 (Abcam, ab150068) was used as a secondary antibody (1:200). Nuclei were stained with DAPI (Vector Laboratories). Vasculature was quantified using ImageJ (National Institutes of Health, Bethesda, MD). Measurements were made 1 high power field (hpf) from the infarct at 20× magnification.

Gaffey A.C., Chen M.H., Venkataraman C.M., Trubelja A., Rodell C.B., Dinh P.V., Hung G., MacArthur J.W., Soopan R.V., Burdick J.A, & Atluri P. (2015). Injectable shear-thinning hydrogels to deliver endothelial progenitor cells, enhance cell engraftment, and improve ischemic myocardium. The Journal of thoracic and cardiovascular surgery, 150(5), 1268-1277.

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