All chemicals used were of analytical grade. D-MEM, αMEM; GMEM, Neurobasal, B27, Geltrex, Acutase, penicillin/streptomycin; NEAA; β-mercaptoethanol and KSR were obtained from Gibco. GDNF and BDNF were from Peprotech. L-glutamine; Na pyruvate; dimethylsulfoxide; Mitomicym C; lipopolysaccharide and p-formaldehyde (PFA) were obtained from Sigma-Aldrich. TNF-α -ELISA Kit was obtained from BD. Triton X-100 and Tween 20 were from Merck. Fetal bovine serum (FBS), and were obtained from Internegocios SA (Argentina). Cyclosporine was from Novartis.
P formaldehyde pfa
Manufactured by Merck Group
P-formaldehyde (PFA) is a chemical compound used in various laboratory applications. It serves as a fixative, preserving the structural integrity of biological samples. PFA is commonly utilized in histology, microscopy, and other scientific procedures that require the stabilization of cellular and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Tnf α elisa kit, by BD (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (2 mentions)
Tween 20, by Merck Group (2 mentions)
Brain derived neurotrophic factor (bdnf), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Merck Group (2 mentions)
Lipopolysaccharide, by Merck Group (2 mentions)
Na pyruvate, by Merck Group (2 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Mitomicym c, by Merck Group (2 mentions)
Cyclosporine, by Novartis (2 mentions)
Axiocam icc3, by Zeiss (1 mentions)
3 protocols using p formaldehyde pfa
1
Comprehensive Reagents for Cell Culture
2
Histological Evaluation of Organ Damage
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Liver, spleen, and lung tissues were fixed in 4% p-formaldehyde (PFA, Sigma) for 24 hr, processed, paraffin-embedded, and sections stained with hematoxylin and eosin using standard methods to evaluate damage. Slides were examined using a Zeiss Axio Scope AX10 microscope with an AxioCamICC3 (Carl Zeiss).
3
Stem Cell Culture and Differentiation
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All chemicals used were of analytical grade. D-MEM, αMEM; GMEM, Neurobasal, B27, Geltrex, Acutase, penicillin/streptomycin; NEAA; βmercaptoethanol and KSR were obtained from Gibco. GDNF and BDNF were from Peprotech. L-glutamine; Na pyruvate; dimethylsulfoxide; Mitomicym C; lipopolysaccharide and p-formaldehyde (PFA) were obtained from Sigma-Aldrich. TNF-α -ELISA Kit was obtained from BD. Triton X-100 and Tween 20 were from Merck. Fetal bovine serum (FBS), and were obtained from Internegocios SA (Argentina). Cyclosporine was from Novartis.
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