Ab9132

Manufactured by Merck Group

Ab9132 is a laboratory equipment product. It is designed to perform a core function for scientific research and analysis. The detailed specifications and intended use of this product are not available.

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Lab products found in correlation

Sc 805, by Santa Cruz Biotechnology (2 mentions) Na931, by Merck Group (2 mentions) F3165, by Merck Group (2 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-GAPDH antibodies (AB9132, (2 citations)

2 protocols using ab9132

Characterization of uL11 Ribosomal Protein Interactions

Cited 2 times

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S2 cells transiently transfected with either pA-uL11-Myc, pA-uL11^K3A-Myc or pA-uL11^K3Y-Myc and pA-FLAG-CortoCD [17 (link)] were harvested after 48 h and washed in Schneider medium at room temperature. Co-immunoprecipitations were performed as described in [31 (link)] without fixation. 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805, Santa Cruz Biotechnology).
Western blot were performed according to standard protocols using mouse anti-Myc (Abcam ab9132; 1/10000) or anti-FLAG (Sigma F3165; 1/5000) as primary antibodies and anti-mouse (Sigma NA931; 1/20000) as secondary antibodies and were revealed using the Supersignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).

Grunchec H., Deraze J., Dardalhon-Cuménal D., Ribeiro V., Coléno-Costes A., Dias K., Bloyer S., Mouchel-Vielh E., Peronnet F, & Thomassin H. (2022). Single amino-acid mutation in a Drosoph ila melanogaster ribosomal protein: An insight in uL11 transcriptional activity. PLoS ONE, 17(8), e0273198.

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Co-Immunoprecipitation of uL11 and CortoCD

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S2 cells transiently transfected with either pA-uL11 K3A -Myc or pA-uL11 K3Y -Myc and pA-FLAG-CortoCD (17) were harvested after 48 h and washed in Schneider medium at room temperature. Co-immunoprecipitations were performed as described in (30) without fixation. 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805, Santa Cruz Biotechnology).
Western blot analyses were performed according to standard protocols with mouse anti-Myc (Abcam ab9132; 1/10000) or anti-FLAG (Sigma F3165; 1/5000) as primary antibodies and anti-mouse (Sigma NA931; 1/20000) as secondary antibodies.

Grunchec H., Deraze J., Dardalhon-Cuménal D., Ribeiro V., Costes A., Dias K., Bloyer S., Mouchel‐Vielh E., Peronnet F., & Thomassin H. (2021). Single amino-acid mutation in a Drosophila melanogaster ribosomal protein: an insight in uL11 transcriptional activity.

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