Ku70 antibody ab83501

Manufactured by Abcam

Ku70 antibody (ab83501) is a protein-specific antibody that recognizes the Ku70 protein. Ku70 is a subunit of the Ku heterodimer, which is involved in the repair of DNA double-strand breaks.

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Lab products found in correlation

Rnase h1 antibody 15606 1 ap, by Proteintech (1 mentions)

Close spelling variants of this product

Ab83501

2 protocols using ku70 antibody ab83501

Synthesis and Use of Antisense Oligonucleotides

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ASOs were synthesized as described previously [14 (link)]. The ASOs used in this study are listed in Supplementary Table S1. RNase H1 antibody (15606-I-AP) was purchased from ProteinTech and Ku70 antibody (ab83501) was from Abcam.

Zhang L., Liang X.H., De Hoyos C.L., Migawa M., Nichols J.G., Freestone G., Tian J., Seth P.P, & Crooke S.T. (2022). The Combination of Mesyl-Phosphoramidate Inter-Nucleotide Linkages and 2′-O-Methyl in Selected Positions in the Antisense Oligonucleotide Enhances the Performance of RNaseH1 Active PS-ASOs. Nucleic Acid Therapeutics, 32(5), 401-411.

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Plasmid Construction and Antibody Acquisition

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Plasmids expressing GST-tagged RNase H1 full-length and different domains, P54nrb, and PSF were synthesized from GenScript and constructed on pGEX-6P1 vector. MBP-tagged RNase H1 was constructed on pMal-C5x vector. Detailed construction is described in Supplementary Table S1. PreScission protease (GE27-0843-01) was purchased from Sigma. Antibodies against P54nrb (sc-376865), PSF (sc-374502) and P32 (sc-48795) were purchased from Santa Cruz Biotechnologies. RNase H1 antibody (15606-1-AP) was purchased from ThermoFisher. Ku70 antibody (ab 83501) and Anti-GST antibody (ab19256) were from Abcam. Secondary anti-mouse or anti-rabbit antibodies were purchased from Bio-Rad. ASOs used in this study are listed in Supplementary Table S2.

Zhang L., Vickers T.A., Sun H., Liang X.H, & Crooke S.T. (2021). Binding of phosphorothioate oligonucleotides with RNase H1 can cause conformational changes in the protein and alter the interactions of RNase H1 with other proteins. Nucleic Acids Research, 49(5), 2721-2739.

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