Log In Sign up
The largest database of trusted experimental protocols

The D8938 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for specialized applications in scientific research and analysis. The core function of the D8938 is to provide precise and reliable performance for the intended use cases. Further details on the specific capabilities and features of this product are not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

Beadbug homogenizer, by Benchmark Scientific (2 mentions)

2 protocols using d8938

1

Brain Tissue Homogenization for rAAV Genome Recovery

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Half of a frozen brain hemisphere (0.3 g approx.) was homogenized with a 2 ml glass homogenizer (Sigma Aldrich; D8938) or a motorized plastic pestle (Fisher Scientific;12-141-361, 12-141-363) (for smaller tissues) or beads using BeadBug Homogenizers (1.5–3.0 mm zirconium or steel beads per manufacturer recommendations) (Homogenizers, Benchmark Scientific, D1032–15, D1032–30, D1033–28) and processed using Trizol as described in our prior work26 (link) (also see Supplementary Note 17). From deep sequencing data analysis, we observed that the amount of tissue processed was sufficient for rAAV genome recovery.
Kumar S.R., Miles T.F., Chen X., Brown D., Dobreva T., Huang Q., Ding X., Luo Y., Einarsson P.H., Greenbaum A., Jang M.J., Deverman B.E, & Gradinaru V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types. Nature methods, 17(5), 541-550.
+ Open protocol
+ Expand
2

Brain Tissue Homogenization for rAAV Genome Recovery

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Half of a frozen brain hemisphere (0.3 g approx.) was homogenized with a 2 ml glass homogenizer (Sigma Aldrich; D8938) or a motorized plastic pestle (Fisher Scientific;12-141-361, 12-141-363) (for smaller tissues) or beads using BeadBug Homogenizers (1.5–3.0 mm zirconium or steel beads per manufacturer recommendations) (Homogenizers, Benchmark Scientific, D1032–15, D1032–30, D1033–28) and processed using Trizol as described in our prior work26 (link) (also see Supplementary Note 17). From deep sequencing data analysis, we observed that the amount of tissue processed was sufficient for rAAV genome recovery.
Kumar S.R., Miles T.F., Chen X., Brown D., Dobreva T., Huang Q., Ding X., Luo Y., Einarsson P.H., Greenbaum A., Jang M.J., Deverman B.E, & Gradinaru V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs for targeting distinct brain cell types. Nature methods, 17(5), 541-550.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!