Gelatine type a

Manufactured by Merck Group

Sourced in Sweden, United States

Gelatine type A is a natural, water-soluble protein derived from the partial hydrolysis of collagen extracted from the skin, bones, and connective tissues of animals. It is commonly used in a variety of applications, including food, pharmaceuticals, and laboratory settings.

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Lab products found in correlation

Domitor vet, by Orion Pharma (1 mentions) Methacrylic anhydride, by Merck Group (1 mentions) μct 80, by Scanco (1 mentions) Advance 400 mhz, by Bruker (1 mentions) Live dead cell staining kit, by Meilun (1 mentions) Sodium hyaluronate, by Yuanye Bio-Technology (1 mentions) 4 6 diamino 2 phenylindole dapi, by Merck Group (1 mentions) Rhodamine phalloidin, by Merck Group (1 mentions) Cck 8 kit, by Meilun (1 mentions)

5 protocols using gelatine type a

Implantation of Microwire Array Electrode

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A microwire array electrode was built in house. The electrode consisted of 29, 12 μm platinum‐irridium wires insulated with parylene C (Paratech, Järfälla, Sweden) and embedded in gelatine type A (2%; Sigma‐Aldrich Co, Saint Louis, MO, USA) for optimal stiffness during insertion into the cortex (Lind et al., 2010). For detailed procedures see Supporting Information Appendix S1.
Seven rats were mounted in a stereotactic frame and implanted with a microwire array electrode in S1. These animals were anaesthetized i.p. with 6.3 mL/kg solution of 1 mg/mL Domitor vet (medetomidin hydrochloride; Orion pharma, Turku, Finland) and 50 mg/mL fentanyl (Braun, Aschaffenburg, Germany). For detailed surgical procedures and postsurgical care see Supporting Information Appendix S1.

Ljungquist B., Jensen T., Etemadi L., Thelin J., Lind G., Garwicz M., Petersson P., Tsanakalis F, & Schouenborg J. (2016). Discrepancies between cortical and behavioural long‐term readouts of hyperalgesia in awake freely moving rats. European Journal of Pain (London, England), 20(10), 1689-1699.

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Fibre-Reinforced GelMA Hydrogels

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GelMA with an 80% degree of methacrylation was synthesized by reacting gelatin from porcine skin (Sigma Aldrich, gelatine Type A, The Netherlands) with methacrylic anhydride (Sigma Aldrich, The Netherlands) following a procedure described elsewhere [17 (link),18 (link)]. Fibre reinforced cylindrical hydrogels with 1 mm height and diameters of 5 or 8 mm were prepared in Polytetrafluoroethylene molds (PTFE; Teflon®). Fibre networks were first cut to the proper size from printed meshes (6×6 cm), and placed into the molds where 10% (w/v) gelMA together with crosslinker solution (Irgacure at 0.1% w/v in PBS) was cast. Samples were then UV crosslinked by exposure to 365 nm UV light for 15 min (UV-Handleuchte lamp A. Germany. Intensity 2.6 mW/cm²). After preparation, high-resolution micro-CT (μCT) analysis was performed using a micro-CT scanner (μCT 80, Scanco Medical AG, Switzerland) at a voltage of 70 kVp, an intensity of 114 μA and an integration time of 300 ms (Gauss filter applied, sigma = 1, support = 0.8 voxel). A water-based contrast agent solution (Ioversol, Optiray 300 TM) was used for staining the GelMA hydrogel during μCT analysis.

Castilho M., Mouser V., Chen M., Malda J, & Ito K. (2019). Bi-layered micro-fibre reinforced hydrogels for articular cartilage regeneration. Acta biomaterialia, 95, 297-306.

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Synthesis of Methacryloyl Gelatin

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Gelatine type A (porcine skin; 300g bloom strength, Sigma-Aldrich) was dissolved in PBS at 10 wt% concentration. Methacrylic anhydride (0.6 g/ml) was added to the gelatine solution and the reaction developed for 1 h at 50°C under constant stirring. The solution was centrifuged and dialysed against deionised water to remove the unreacted methacrylic anhydride. The GelMA solution pH was adjusted to 7.4, followed by sterile filtration (0.22 μm filter) and lyophilisation. The degree of modification for methacryloyl substitution was quantified to be 60% using 1H-proton nuclear magnetic resonance spectroscopy (Bruker Advance 400 MHz).

Cidonio G., Alcala-Orozco C.R., Lim K.S., Glinka M., Mutreja I., Kim Y.H., Dawson J.I., Woodfield T.B.F, & Oreffo R.O.C. (2019). Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinks. Biofabrication, 11(3).

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Trypanosome Immobilization Technique

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10⁷ trypanosomes were resuspended in 20 μl TDB. 3 μl of the cell suspension was mixed with 5 μl of 10% (w/v) type-A gelatine in TDB (from porcine skin; Sigma-Aldrich) and pipetted between two cover slips. The coverslips were mounted in a temperature-controlled microscope sample holder. The experimental conditions were optimised for transient immobilisation of trypanosomes at 20 °C. At this temperature the cells are metabolically largely unaffected and trapping of the parasites up to at least 3 hours in gelatine does not impair their viability. On release from gelatine immobilization cells proliferated normally.

Hartel A.J., Glogger M., Guigas G., Jones N.G., Fenz S.F., Weiss M, & Engstler M. (2015). The molecular size of the extra-membrane domain influences the diffusion of the GPI-anchored VSG on the trypanosome plasma membrane. Scientific Reports, 5, 10394.

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Gelatine-Hyaluronate Hydrogel Synthesis

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Type A gelatine was purchased from Sigma‒Aldrich (USA). Aniline, (3-(dimethylamino)propyl)-N′-ethylcarbodiimide hydrochloride (EDC), sodium periodate purchased from Aladdin Co., Ltd, China. Sodium hyaluronate was purchased from Shanghai Yuanye Biotechnology Co., Ltd, China. Ethylene glycol and sodium dihydrogen phosphate were purchased from Beijing Chemical Co., Ltd, China. The CCK-8 kit and live-dead cell staining kit were purchased from Dalian Meilun Biotechnology Co., Ltd, China. 4,6-Diamino-2-phenylindole (DAPI) and rhodamine-phalloidin were purchased from Sigma‒Aldrich (USA). The reagents for cell experiments were purchased from Abcam (UK). Haematoxylin and eosin (H&E) staining kits were purchased from Shanghai Biyuntian Experimental Reagent Co., Ltd, China. Fast blue staining kits were purchased from Solarbio Co., Ltd, China.

Liu T., Zhang Q., Li H., Cui X., Qi Z, & Yang X. (2023). An injectable, self-healing, electroconductive hydrogel loaded with neural stem cells and donepezil for enhancing local therapy effect of spinal cord injury. Journal of Biological Engineering, 17, 48.

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