Cells harvested from colony forming assay were washed in PBS and lysed in TRI Reagent (Zymo Research). RNA extraction was performed using Direct-zol RNA Kits (Zymo Research) according to the protocol of the manufacturer. RNA concentration and quality were determined with the Agilent 2100 Bioanalyzer system using the Eukaryote Total RNA Pico Assay kit. RNA sequencing libraries were prepared using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit according to the manufacturer’s instructions. The quality and concentration of final libraries were assessed using Agilent 2100 Bioanalyzer system and Qubit DNA HS assay, respectively.
Total rna pico assay kit
Manufactured by Agilent Technologies
The Total RNA Pico Assay kit is a product designed to quantify and assess the quality of total RNA samples. It enables the analysis of low-input RNA samples, such as those from single cells or microdissected tissues, using sensitive fluorometric detection methods.
Automatically generated - may contain errors
Lab products found in correlation
Qubit dna hs assay, by Agilent Technologies (2 mentions)
Smarter stranded total rna seq kit v2 pico input mammalian kit, by Takara Bio (2 mentions)
Direct zol rna kit, by Zymo Research (2 mentions)
Agilent bioanalyzer 2100 system, by Agilent Technologies (2 mentions)
Tri reagent, by Zymo Research (2 mentions)
2 protocols using total rna pico assay kit
1
RNA Extraction and Sequencing Library Prep
2
RNA Extraction and Sequencing Library Prep
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Cells harvested from colony forming assay were washed in PBS and lysed in TRI Reagent (Zymo Research). RNA extraction was performed using Direct-zol RNA Kits (Zymo Research) according to the protocol of the manufacturer. RNA concentration and quality were determined with the Agilent 2100 Bioanalyzer system using the Eukaryote Total RNA Pico Assay kit. RNA sequencing libraries were prepared using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit according to the manufacturer’s instructions. The quality and concentration of final libraries were assessed using Agilent 2100 Bioanalyzer system and Qubit DNA HS assay, respectively.
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