Primary human hair DPCs (hDPCs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA) as primary cells and maintained in mesenchymal stem cell (MSC) medium (ScienCell Research Laboratories), 5% (w/v) FBS, 1% (w/v) penicillin, 1% (w/v) mesangial cell growth supplement (ScienCell Research Laboratories) under a 5% CO2, humidified environment at 37 °C. For optimal results, we followed the cell culture recommendations of the supplier. Media, FBS, and other chemicals were obtained from ScienCell2 (link).
Hdpcs
Manufactured by ScienCell
Sourced in United States
HDPCs are primary human dermal papilla cells. They are isolated from human dermal papilla tissue and cultured in vitro. HDPCs play a crucial role in hair follicle development and maintenance.
Automatically generated - may contain errors
2 protocols using hdpcs
1
Primary Human Hair Dermal Papilla Cell Culture
2
Culturing Human Hair Dermal Papilla Cells
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Human hair DPCs (hDPCs) were purchased from ScienCell (Cat. #2400, CA, USA). Cells were cultured in mesenchymal stem cell medium (Cat. #7501, ScienCell) containing 10% FBS, 1% mesenchymal stem cell growth supplement (MSCGS, Cat. No. 7552, ScienCell), and 1% penicillin–streptomycin. The immortalized human keratinocyte (HaCaT) cells were purchased from Cell Line Service (Eppelhein, Germany). Cells were cultured in high glucose DMEM containing 10% FBS and 1% penicillin–streptomycin. Both cells were cultured and maintained in a 5% CO2 humidified incubator at 37 °C.
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