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On targetplus ask1 sirna

Manufactured by Horizon Discovery
Sourced in Australia

The ON-TARGETplus ASK1 siRNA is a gene silencing reagent designed to target the ASK1 (Apoptosis Signal-regulating Kinase 1) gene. It is a double-stranded RNA molecule that can be used to temporarily suppress the expression of the ASK1 gene in cell-based experiments.

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2 protocols using on targetplus ask1 sirna

1

ASK1 Regulation of Cell Signaling

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Antibodies against rabbit total and phospho-apoptosis signal-regulating kinase 1 (ASK1), phospho-p42/p44, phospho-p38, phospho-JNK1/2 antibodies were from Cell Signaling Technology (Beverly, MA, USA). IRDye 680 or 800 secondary antibodies were from Rockland (Gilbertsville, PA, USA). CyQUANT cell proliferation assay kit was purchased from Life Technologies (Grand Island, NY, USA). TC ASK 10, an ASK1 inhibitor was purchased from Tocris Biosciences, USA, recombinant TGFβ1 was obtained from R&D Systems, Minneapolis, MN, USA. ON-TARGETplus ASK1 siRNA was purchased from Dharmacon, Mulgrave, Victoria, Australia. Lipofectamine RNAiMAX was purchased from Invitrogen, Australia. All other chemicals were of analytical grade and were obtained from Sigma Aldrich, USA.
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2

ASK1 Regulation of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against rabbit total and phospho-apoptosis signal-regulating kinase 1 (ASK1), phospho-p42/p44, phospho-p38, phospho-JNK1/2 antibodies were from Cell Signaling Technology (Beverly, MA, USA). IRDye 680 or 800 secondary antibodies were from Rockland (Gilbertsville, PA, USA). CyQUANT cell proliferation assay kit was purchased from Life Technologies (Grand Island, NY, USA). TC ASK 10, an ASK1 inhibitor was purchased from Tocris Biosciences, USA, recombinant TGFβ1 was obtained from R&D Systems, Minneapolis, MN, USA. ON-TARGETplus ASK1 siRNA was purchased from Dharmacon, Mulgrave, Victoria, Australia. Lipofectamine RNAiMAX was purchased from Invitrogen, Australia. All other chemicals were of analytical grade and were obtained from Sigma Aldrich, USA.
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