Gfp cuff

Manufactured by Vienna Drosophila Resource Center

The GFP-Cuff is a specialized laboratory instrument designed to visualize and analyze the expression of green fluorescent protein (GFP) in Drosophila samples. It provides a reliable and efficient method for researchers to observe and quantify GFP-tagged proteins within the model organism.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using gfp cuff

Drosophila Transgenic Stocks for ChIP-seq

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following stocks were used: bam^Δ86 (BDSC5427), bam^Df (BDSC27403), C(1)RM (BDSC9460), nos-Gal4 (BDSC4937), UASp-shRhi (BDSC35171), iso-1 (BDSC2057), GFP-Cid (BDSC25047), GFP-D1 (BDSC50850) were obtained from Bloomington Drosophila Stock Center; GFP-Rhi (VDRC313340), GFP-Del (VDRC313271), GFP-Cuff (VDRC313269), moon^Δ1 (VDRC313735), moon^Δ28 (VDRC313738), Bam-GFP (VDRC318001), w¹¹¹⁸ (VDRC60000) were obtained from Vienna Drosophila Resource Center; tj-Gal4 (DGRC104055) was obtained from Kyoto Stock Center; rhi², rhi^KG and UASp-GFP-Rhi were gifts of William Theurkauf; del^WK36, del^HN56, cuff^QQ37and cuff^WM25 were gifts of Trudi Schüpbach; GFP-Vasa (gift of Paul Lasko), copiaLTR-lacZ (gift of Elena Pasyukova), GFP-Moon (gift of Peter Andersen), Sa-GFP (gift of Xin Chen). UASp-mKate2 was described before [3 (link)]. GFP-tagged Rhi, Del, Cuff and Moon are previously described transgenes constructed by inserting N-terminal GFP into genomic BACs via recombineering [2 (link),8 (link)]. XO males were generated by crossing GFP-Rhi males to C(1)RM females. To perform GFP-Rhi ChIP, bam^Δ86 and GFP-Rhi were recombined.

Chen P., Luo Y, & Aravin A.A. (2021). RDC complex executes a dynamic piRNA program during Drosophila spermatogenesis to safeguard male fertility. PLoS Genetics, 17(9), e1009591.

+ Open protocol

+ Expand

Drosophila Germline Stem Cell Genetics

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following stocks were used: bam ∆86 (BDSC5427), bam Df (BDSC27403), C(1)RM (BDSC9460), nos-Gal4 (BDSC4937), UASp-shRhi (BDSC35171), iso-1 (BDSC2057) were obtained from Bloomington Drosophila Stock Center; GFP-Rhi (VDRC313340), GFP-Del (VDRC313271), GFP-Cuff (VDRC313269), moon ∆1 (VDRC313735), moon ∆28 (VDRC313738), Bam-GFP (VDRC318001) were obtained from Vienna Drosophila Resource Center; tj-Gal4 (DGRC104055) was obtained from Kyoto Stock Center; rhi 2 , rhi KG and UASp-GFP-Rhi were gifts of William Theurkauf; del WK36 , del HN56 , cuff QQ37 and cuff WM25 were gifts of Trudi Schüpbach; GFP-Vasa (gift of Paul Lasko), copiaLTR-lacZ (gift of Elena Pasyukova), GFP-Moon (gift of Peter Andersen), Sa-GFP (gift of Xin Chen). UASp-mKate2 was described before (Chen et al., 2016) (link). XO males were generated by GFP-Rhi males crossed to C(1)RM females. bam ∆86 and GFP-Rhi were recombined for testis ChIP.

Chen P., Luo Y., & Aravin A.A. (2020). RDC complex executes a dynamic piRNA program duringDrosophilaspermatogenesis to safeguard male fertility.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!