Dl threo pdmp

Ultra-pure water was obtained by a Milli-Q plus system from Millipore (Bedford, MA, USA). LC–MS grade acetonitrile (MeCN), methanol (MeOH), isopropanol (IPA) and ammonium acetate were purchased from Scharlab (Barcelona, Spain), while chloroform and Phosphate Buffer Saline (PBS) tabs were purchased from Sigma-Aldrich (St Louis, MO, USA). Major Mix IMS/TOF calibration kit and Leukin-Enkephalin (Leu-Enk) solution from Waters (Manchester, UK) were employed for external and internal calibration of the mass spectrometer. Lipidomix® kit was purchased from AVANTI Polar (Alabaster, AL, USA). Osimertinib was provided by AstraZeneca (Milan, Italy). PDMP, namely, DL-threo PDMP (a mixture of D-threo-(1R,2R)-PDMP and L-threo-(1S,2S)-PDMP isomers) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Stock solutions were prepared at a convenient concentration by dissolving the required amount of the titled compound in DMSO (Sigma Aldrich), aliquoted to reduce the freeze–thaw cycles, and diluted in fresh medium before use. Final DMSO concentration in medium never exceeded 0.1% (v/v).

2-AG, AEA, noladin, EPEA and DL-threo-PDMP were purchased from Cayman Chemical (Ann Arbor, Michigan, USA). Cholesterol, AA, C17iso fatty acid, Dubelcco´s medium (DMEM) and antioxidant BHT were purchased from Sigma (Sigma-Aldrich, St. Louis, Missouri, USA). mmPEGC-C22^{10 (link)}, Δ⁴-DA and Δ⁷-DA were provided by Prof. H.-J. Knölker^{43 (link)–45 (link)}.
The worm strain N2 Bristol and the single mutants daf-7(1372), fat-3(ok1126), fat-4 (ok958), ncr-1;ncr-2 (JT10800) were obtained from Caenorhabditis Genetics Center (CGC). daf-9(dh6);dhEx24 was provided by Prof. A. Antebi.