Anti p34 arc arpc2

The following primary mouse monoclonal antibodies were used: anti-E-cadherin, clone 36; anti-β-catenin, clone 14; anti-paxillin, clone 349 (BD Transduction Labs, Franklin Lakes, NJ, USA); anti-zyxin, clone 164D4 (Synaptic Systems, Göttingen, Germany); anti-β-actin, clone 4C2 (Merck, Darmstadt, Germany); anti-vinculin, clone hVin1 (Sigma, Merck, Darmstadt, Germany); anti-α-actinin-1, clone BM-75.2 (Sigma, Merck); anti-α-catenin, clone 15D9 (Enzo Life Sciences, Farmingdale, NY, USA); rabbit polyclonal anti-p34-Arc/ARPC2 (Upstate, Merck, Darmstadt, Germany); anti-pY654-β-catenin (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). The secondary goat anti-mouse IgG, IgG1, IgG2a, IgG2b or anti-rabbit IgG antibodies conjugated with AlexaFluor488, 594 or 647 (Jackson Immunoresearch, West Grove, PA, USA) were used. AlexaFluor488-conjugated phalloidin (Molecular Probes, Eugene, OR, USA) or TRITC-conjugated phalloidin (Fluka Chemie, Buchs, Switzerland) was added to the secondary antibodies. For Western blotting, goat anti-mouse and anti-rabbit IgG antibodies conjugated to horse radish peroxidase (Jackson Immunoresearch) were used. Other reagents were obtained from Sigma, Merck.