Dionex ics 3000 dp lc system

The quantification of sugars and sugar alcohols was conducted using a high-performance anion-exchange liquid chromatography system, Dionex ICS 3000 DP LC (Dionex, Sunnyvale, CA, USA), with pulsed amperometric detection. For the organic acids analysis, the Dionex ICS 3000 DP LC system was coupled with a conductivity detector. The performance of the ion chromatography device and the determination conditions for the quantification of sugars, sugar alcohols, and organic acids are described in our previous work [39 (link)].

A high-performance anion-exchange liquid chromatography system with pulsed amperometric detection was used to analyze sugars and sugar alcohols. The chromatographic measurement was performed using the Dionex ICS 3000 DP LC system (Dionex, Sunnyvale, CA, USA), which is equipped with a quaternary gradient pump and electrochemical detector consisting of Au as the working electrode and Ag/AgCl as the reference electrode, an autosampler (AS-DV) and Chromeleon software (Chromatography Workstation and Chromeleon Chromatography Management Software—version 6.7). All separations were performed on the Carbo Pac PA100 column (4 × 250 mm (analytical) and 4 × 50 mm (guard); Dionex) thermostatted to 30 °C. The mobile phase flow rate was 0.7 mL/min, and the composition of mobile phase was changed during the analysis in the following order (gradient elution): −20–5 min = 15% 300 mM NaOH; 5–12 min = 15% 300 mM NaOH and 2% 500 mM NaOAc; 12–20 min = 15% 300 mM NaOH and 4% 500 mM NaOAc; 20–30 min = 20% 300 mM NaOH and 20% 500 mM NaOAc; the rest up to 100% is ultrapure water. The total running time of the analyses was 30 min.