Pectinase from Aspergillus aculeatus (Pectinex® Ultra Clear, a blend of pectinases, hemicellulases and arabinases with activity of 8600 PGNU/g) and (Pectinex® Ultra SP-L, a blend of pectinases, hemicellulases and beta-glucanases with activity of 3300 PGNU/g), cellulase from Trichoderma reesei (Celluclast® 1.5L, a cellulase aqueous solution with activity of ≥700 units/g) were obtained from Sigma Aldrich (Darmstadt, Germany). Information about the optimal conditions of these enzymes as declared by the producer can be found in Table 1 . Microcrystalline cellulose Avicel® PH-101, pectin from citrus peel, 3,5-dinitro salicylic acid, D-(+)-galacturonic acid monohydrate and D-(+)-glucose were purchased from Sigma Aldrich (Darmstadt, Germany). Sodium carbonate anhydrous, citric acid, sodium hydroxide, and L (+)-potassium sodium tartrate tetrahydrate were purchased from VWR Chemicals (Leuven, Belgium), while di-sodium hydrogen phosphate anhydrous, sodium acetate trihydrate, and acetic acid 99–100 % were obtained from Chem-Lab (Zedelgem, Belgium).
Conditions range of activity of the used enzymes as declared by the producer.
| Enzyme | pH range of activity | Temperature range of activity |
|---|---|---|
| Pectinex® Ultra Clear | 1.8–3.0 | 25–40 °C |
| Pectinex® Ultra SP-L | 2.8–6.5 | 15–55 °C |
| Celluclast® 1.5L | 4.0–6.0 | 50–60 °C |