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Atto 488 labeled guanosine diphosphate eda gdp atto 488

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ATTO 488-labeled guanosine diphosphate (EDA-GDP-ATTO 488) is a fluorescently labeled nucleotide derivative. It consists of the guanosine diphosphate (GDP) molecule covalently linked to the ATTO 488 fluorescent dye via an ethylenediamine (EDA) linker. This product can be used for various biochemical and biophysical applications that require the detection and/or tracking of GDP-binding proteins or processes.

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Lab products found in correlation

Guanosine triphosphate gtp, by Merck Group (2 mentions) Bodipy gdp, by Thermo Fisher Scientific (2 mentions) Atto 647n maleimide, by Jena Biosciences (2 mentions) Eda gppnp atto 488, by Jena Biosciences (2 mentions) Guanosine diphosphate gdp, by MP Biomedicals (2 mentions) Biotinylated anti chicken igm, by Merck Group (2 mentions) Tr dhpe, by Thermo Fisher Scientific (2 mentions) Cy5 labeled streptavidin, by Thermo Fisher Scientific (2 mentions) Bodipy gtp, by Thermo Fisher Scientific (2 mentions)

2 protocols using atto 488 labeled guanosine diphosphate eda gdp atto 488

1

Purification and Labeling of H-Ras and SOS Proteins

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H-RasC118S C181 (H-Ras construct containing residues 1–181 with a single cysteine at position C181 used for coupling to the bilayer, henceforth simply H-Ras), SOSCat cys-lite (residues 566–1049 with following mutations: C838A, C635A, C980S, E718C), SOSDPC (residues 198–1049), SOSHDPC (residues 1–1049), and SOSHDPC(R552G) (residues 1–1049 with R552G) of human SOS1 were expressed in E. Coli and purified as previously described22 (link). Lipids were purchased from Avanti (Alabaster, AL). TR-DHPE, BODIPY-GDP and BODIPY-GTP were purchased from Invitrogen (Carlsbad, CA). ATTO 647N-maleimide, ATTO 488-labeled guanosine diphosphate (EDA-GDP-ATTO 488) and EDA-GppNp-ATTO 488 (non-hydrolyzeable analog of guanosine triphosphate) were purchased from Jena Bioscience (Jena, Germany). Guanosine triphosphate (GTP) was purchased from Sigma-Aldrich (Saint Louis, MO) and guanosine diphosphate (GDP) was purchased from MP biomedicals (Santa Ana, CA). Biotinylated anti-Chicken IgM was purchased from Sigma (#SAB3700240) and Cy5 labeled streptavidin was from Life Technologies (#43–4316).
Christensen S.M., Tu H.L., Jun J.E., Alvarez S., Triplet M.G., Iwig J.S., Yadav K.K., Bar-Sagi D., Roose J.P, & Groves J.T. (2016). One-way membrane trafficking of SOS in receptor-triggered Ras activation. Nature structural & molecular biology, 23(9), 838-846.
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2

Purification and Labeling of H-Ras and SOS Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
H-RasC118S C181 (H-Ras construct containing residues 1–181 with a single cysteine at position C181 used for coupling to the bilayer, henceforth simply H-Ras), SOSCat cys-lite (residues 566–1049 with following mutations: C838A, C635A, C980S, E718C), SOSDPC (residues 198–1049), SOSHDPC (residues 1–1049), and SOSHDPC(R552G) (residues 1–1049 with R552G) of human SOS1 were expressed in E. Coli and purified as previously described22 (link). Lipids were purchased from Avanti (Alabaster, AL). TR-DHPE, BODIPY-GDP and BODIPY-GTP were purchased from Invitrogen (Carlsbad, CA). ATTO 647N-maleimide, ATTO 488-labeled guanosine diphosphate (EDA-GDP-ATTO 488) and EDA-GppNp-ATTO 488 (non-hydrolyzeable analog of guanosine triphosphate) were purchased from Jena Bioscience (Jena, Germany). Guanosine triphosphate (GTP) was purchased from Sigma-Aldrich (Saint Louis, MO) and guanosine diphosphate (GDP) was purchased from MP biomedicals (Santa Ana, CA). Biotinylated anti-Chicken IgM was purchased from Sigma (#SAB3700240) and Cy5 labeled streptavidin was from Life Technologies (#43–4316).
Christensen S.M., Tu H.L., Jun J.E., Alvarez S., Triplet M.G., Iwig J.S., Yadav K.K., Bar-Sagi D., Roose J.P, & Groves J.T. (2016). One-way membrane trafficking of SOS in receptor-triggered Ras activation. Nature structural & molecular biology, 23(9), 838-846.
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