Insulin levels and adiponectin levels were measured by Ultra-Sensitive Mouse Insulin Enzyme-Linked Immunosorbent Assay (ELISA) kit (Mercodia) and adiponectin kits (Abcam) according to the manufacturer’s instruction. The plates were read with a microplate reader (SpectraMax MS; Molecular Devices). The coefficient of variance between replicates was 4.5% and 4.3%, and the corresponding detection range was 1.56 to 100 ng/mL and 62.5 to 4000 pg/mL. Blood samples were collected from tail. For measurement of insulin and adiponectin levels in SM, tissues were first lysed with RIPA lysis buffer (Biovision) and centrifuged at 12 000g at 4 °C for 20 minutes. The supernatants were collected for analysis.
Adiponectin kits
Manufactured by Abcam
Sourced in United States
Adiponectin kits are laboratory tools used to measure the levels of the adiponectin protein in biological samples. Adiponectin is a hormone produced by adipose tissue and plays a role in regulating glucose and lipid metabolism. These kits provide a quantitative assessment of adiponectin concentration, which can be useful for research purposes.
Automatically generated - may contain errors
2 protocols using adiponectin kits
1
Insulin and Adiponectin Measurement in Mice
2
Serum Biomarkers Quantification Protocol
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Glycated hemoglobin (HbA1c) in the fasting serum was measured in duplicate using a commercial assay kit (Crystal Chem, IL, USA) following the kit instructions. The concentration of serum insulin, leptin, and adiponectin was also measured using the ELISA kits, with the rat insulin kits being obtained from Mercodia (Uppsala, Sweden), leptin kits from R&D Systems (Minneapolis, MN, USA) and adiponectin kits from Abcam Inc. (Toronto, On, Canada). The coefficient of variance between replicates was 7.2%, 5.1%, and 4.3% and detection range was D r a f t 10 1.56-100 ng/ml, 0.15-5.5 µg/ml, and 62.5-4,000 pg/ml for adiponectin, insulin and leptin ELISA kits, respectively. All samples were within the detection range except those for adiponectin for which a dilution factor of 1:400 was applied.
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