Leica mz liii

Soybean oil emulsions possessing an o/w ratio from 20/80 to 50/50 and a ChNC concentration of 0.05–0.5 wt % were formed using ultrasonication with a titanium sonotrode for 2 min with a pulse sequence of 0.5/0.5 s on/off and amplitude of 80% operating at 24 kHz frequency and 320 W power processor (UP400S, Hielscher, Teltow, Germany). The oil droplet size was measured using a laser diffraction particle size analyzer (LS 13 320; Beckman Coulter, Indianapolis, IN, USA). The morphology of the oil droplet was observed and imaged using a Leica MZ LIII stereomicroscope (Leica Microsystems Ltd., Heerbrugg, Switzerland), with a 10× objective lens from a drop of emulsion dipped onto a microscope slide.

The
soybean O/W emulsions stabilized by ACNFs were prepared using an Ultra-Turrax
mixer (IKA T25, Germany) at 8000 rpm for 10 min. The oil-to-water
ratio was maintained at 1:10 throughout the study. The ACNFs (0.1%,
w/w) were added to the water phase before the oil was added. An O/W
emulsion without ACNFs addition was used as a reference. The size
of oil droplets was analyzed with a laser-diffraction particle size
analyzer (LS 13 320, Beckman Coulter, USA), and three parallel measurements
were performed. Droplet sizes of oil were observed and imaged using
a Leica MZ LIII stereomicroscope (Leica Microsystems Ltd., Heerbrugg,
Switzerland) to visualize possible aggregation after dispersion.